Simple Plex Human LBP Cartridge

Catalog #: SPCKB-PS-000508 Datasheet / COA / SDS

Simple Plex Human LBP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000508
SPCKA-PS-000508
SPCKC-PS-000508

Control Products Available

898201: Simple Plex Control for Human LBP

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All LBP Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human LBP Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

6.88 pg/mL

Assay Range

98.3 - 150,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human LBP.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human LBP in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	37	37
Mean (pg/mL)	1025	51532	1036	51807
Standard Deviation	71.4	3306	116	4349
CV%	7	6.4	11.1	8.4

Recovery

Recovery was not necessary as LBP shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human LBP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human LBP Simple Plex Assay Standard Curve

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Human LBP Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LBP

Lipopolysaccharide-binding protein (LBP), a 58kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins that includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipidA portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyzes the binding of LPS monomers to CD14, and promotes LPS-induced immune response.

Long Name:

Lipopolysaccharide-binding Protein

Entrez Gene IDs:

3929 (Human); 16803 (Mouse)

Alternate Names:

LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; LPS-binding protein; MGC22233