Simple Plex Human Osteoprotegerin/OPG Cartridge

Catalog #: SPCKB-PS-000521 Datasheet / COA / SDS

Simple Plex Human Osteoprotegerin/TNFRSF11B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000521
SPCKA-PS-000521
SPCKC-PS-000521

Control Products Available

898209: Simple Plex Control for Human Osteoprotegerin/TNFRSF11B

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All Osteoprotegerin/TNFRSF11B Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Osteoprotegerin/OPG Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

1.17 pg/mL

Assay Range

4.2 - 40,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human OPG.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human OPG in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	26	26
Mean (pg/mL)	231	12102	242	11197
Standard Deviation	16.3	976	23.5	1133
CV%	7.1	8.1	9.7	10.1

Recovery

Recovery was not necessary as OPG shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human OPG were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human Osteprotegerin Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin (OPG), also known as OCIF and TNFRSF11B, is a secreted TNF superfamily receptor that regulates bone density. OPG is widely expressed and constitutively released as a homodimer by mesenchymal stem cells, fibroblasts, and endothelial cells. OPG functions as a decoy receptor for TRANCE/RANK L and TRAIL. OPG regulates the cell surface expression of TRANCE and its ability to stimulate osteoclastogenesis. Dysregulation of OPG/TRANCE interactions can lead to juvenile Paget’s disease, osteoporosis, bone loss, and vascular calcification.

Entrez Gene IDs:

4982 (Human); 18383 (Mouse)

Alternate Names:

OCIF; OCIFMGC29565; OPG; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b