Simple Plex Human TFF3 Cartridge

Catalog #: SPCKB-PS-000530 Datasheet / COA / SDS

Simple Plex Human TFF3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000530
SPCKA-PS-000530
SPCKC-PS-000530

Control Products Available

898416: Simple Plex Control for Human TFF3

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All TFF3 Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human TFF3 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

2.41 pg/mL

Assay Range

13.1 - 20,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human TFF3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human TFF3 in serum and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	17	19
Mean (pg/mL)	137	7796	114	7221
Standard Deviation	8.48	514	14.4	647
CV%	6.2	6.6	12.6	9

Recovery

Recovery was not necessary as TFF3 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human TFF3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human TFF3 Simple Plex Assay Standard Curve

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Human TFF3 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TFF3

TFF3 (Trefoil Factor 3), also known as ITF and P1.B, is a peptide that plays an important role in epithelial regeneration and wound healing. It can form disulfide-linked dimers or associate into disulfide-linked complexes with the intestinal mucous proteins FCGBP and MUC-2. TFF3 is expressed by epithelial goblet cells in the respiratory tract, biliary and breast ducts, small and large intestine, and cardia of the stomach as well as by chondrocytes during bone development. TFF3 is upregulated in response to gastrointestinal epithelial disruptions and promotes epithelial wound healing. It enhances tumor cell invasion and metastasis by supporting hypoxia-induced VEGF upregulation.

Long Name:

Trefoil Factor 3

Entrez Gene IDs:

7033 (Human); 21786 (Mouse); 25563 (Rat)

Alternate Names:

HITF, human intestinal trefoil factor10Intestinal trefoil factor; ITF; TFF3; TFI; trefoil factor 3 (intestinal)