Simple Plex Human Trappin-2/Elafin Cartridge

Catalog #: SPCKB-PS-000228 Datasheet / COA / SDS

Simple Plex Human Trappin-2/Elafin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000228
SPCKA-PS-000228
SPCKC-PS-000228

Control Products Available

898343: Simple Plex Control for Human Trappin-2/Elafin

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Trappin-2/Elafin Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

1.12 pg/mL

Assay Range

3.8 - 14,400 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Trappin-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Trappin-2 in serum and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	18	18
Mean (pg/mL)	92	4838	102	4665
Standard Deviation	8.1	427	13	339
CV%	8.8	8.8	12.8	7.3

Recovery

Recovery was not necessary as Trappin-2 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human Trappin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

Human Trappin-2 Simple Plex Assay Standard Curve

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Human Trappin-2/Elafin Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Trappin-2/Elafin

Trappin-2 is a member of the trappin family that includes the SLPI (secretory leukocyte protease inhibitor) protein. Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leukoproteinase) are alternative names for trappin-2 and reflect its protease targets. However, Elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule.

Long Name:

Elastase Inhibitor, Transglutaminase Substrate, Serine Protease

Entrez Gene IDs:

5266 (Human)

Alternate Names:

cementoin; Elafin; Elastase-specific inhibitor; ESI; ESIWAP four-disulfide core domain protein 14; Peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI3; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); Protease inhibitor WAP3; SKALP; SKALPELAFIN; Skin-derived antileukoproteinase; Trappin2; Trappin-2; WAP four-disulfide core domain 14; WAP3MGC13613; WFDC14elafin