Simple Plex Mouse IFN-gamma Cartridge

Catalog #: SPCKB-MP-001945 Datasheet / COA / SDS

Simple Plex Mouse IFN-gamma assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

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SPCKB-MP-001945
SPCKA-MP-001945
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Control Products Available

898652: Simple Plex Control for Mouse IFN-gamma

Simple Plex Cartridge Kit for 72 samples, 1 analyte for use with Mouse Plasma/Serum.

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse IFN-gamma Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Serum (25 uL), Mouse EDTA Plasma (25 uL), Mouse Heparin Plasma (25 uL)

Sensitivity

0.48 pg/mL

Assay Range

0.9 - 9,000 pg/mL (Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)

Specificity

Natural and recombinant mouse IFN-gamma.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse IFN-gamma in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	51	51
Mean (pg/mL)	44.4	2296	51.7	2486
Standard Deviation	1.33	205	5.35	263
CV%	3	8.9	10.4	10.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Mouse EDTA Plasma (n=4)	118	112-123
Mouse Heparin Plasma (n=4)	116	112-122
Mouse Serum (n=4)	114	107-118

Linearity

Samples containing and/or spiked with high concentrations of mouse IFN- gamma were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Mouse IFN-gamma Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name:

Interferon gamma

Entrez Gene IDs:

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Alternate Names:

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma