Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge

Catalog #: SPCKB-MP-000584 Datasheet / COA / SDS

Simple Plex Mouse/Rat CCL2/JE/MCP-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-MP-000584
SPCKA-MP-000584
SPCKC-MP-000584

Control Products Available

898305: Simple Plex Control for Mouse/Rat CCL2/JE/MCP-1

Simple Plex Cartridge Kit for 72 samples, 1 analyte for use with Mouse Plasma / Serum.

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Mouse/Rat CCL2/JE/MCP-1 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Cell Culture Supernates (5 uL), Rat Cell Culture Supernates (1 uL), Mouse Serum (25 uL), Rat Serum (5 uL), Mouse EDTA Plasma (25 uL), Rat EDTA Plasma (25 uL), Mouse Heparin Plasma (25 uL), Rat Heparin Plasma (25 uL), Mouse Cerebrospinal Fluid (25 uL)

Sensitivity

0.16 pg/mL

Assay Range

0.7 - 1,090 pg/mL (Mouse Cell Culture Supernates, Rat Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma, Mouse Cerebrospinal Fluid)

Specificity

Natural and recombinant mouse/rat MCP-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse/rat MCP-1 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and mouse cerebrospinal fluid (CSF)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Cell Culture Supernates, Rat Cell Culture Supernates, Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Mouse Heparin Plasma, Rat Heparin Plasma, Mouse Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample
n	16	16	54	54
Mean (pg/mL)	331	6.41	313	6.6
Standard Deviation	20.2	0.484	27.4	0.697
CV%	6.1	7.5	8.8	10.6

Recovery

Recovery was not necessary as mouse/rat MCP-1 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat MCP-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Mouse/Rat MCP-1 Simple Plex Standard Curve

Product Datasheets

Product Datasheet

COA

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL2/JE/MCP-1

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.

Entrez Gene IDs:

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Alternate Names:

C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF