Simple Plex Neuro Panel 1
Simple Plex Neuro Panel 1 Summary
The Simple Plex Human Multianalyte Assay is for the detection of human GFAP 2nd Gen and NF-L in serum and plasma (EDTA/Heparin).| Sample Type & Volume Required Per Well | Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL) |
| Sensitivity | GFAP 2nd Gen 0.64 pg/mL; NF-L 1.09 pg/mL |
| Assay Range | GFAP 2nd Gen 2.52 - 9,600 pg/mL; NF-L 2.7 - 10,290 pg/mL |
Product Specifications
| Reactivity | Human |
| Specificity | Natural and recombinant human GFAP 2nd Gen and NF-L. |
| Cross-reactivity | <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules. |
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent
| Intra-Assay Precision | Inter-Assay Precision | ||||
|---|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 | |
| GFAP 2nd Gen | n | 16 | 16 | 62 | 59 |
| Mean (pg/mL) | 75.1 | 3705 | 76.3 | 3529 | |
| Standard Deviation | 3.54 | 218 | 6.08 | 257 | |
| CV (%) | 4.7 | 5.9 | 8 | 7.3 | |
| NF-L | n | 16 | 16 | 48 | 48 |
| Mean (pg/mL) | 76.6 | 3309 | 80.5 | 3476 | |
| Standard Deviation | 3.64 | 154 | 8.35 | 267 | |
| CV (%) | 4.8 | 4.7 | 10.4 | 7.7 | |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % | |
|---|---|---|---|
| GFAP 2nd Gen | EDTA Plasma (n=4) | 94 | 90-98 |
| Heparin Plasma (n=4) | 95 | 90-102 | |
| Serum (n=4) | 91 | 86-100 | |
| Cell Culture Media (n=2) | 110 | 110-111 | |
| NF-L | EDTA Plasma (n=4) | 93 | 86-99 |
| Heparin Plasma (n=4) | 101 | 93-106 | |
| Serum (n=4) | 108 | 95-115 | |
| Cell Culture Supernates (n=5) | 12 | 110-115 |
Specifications
Product Datasheets
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