Simple Plex Neuroinflammation Panel 1
Simple Plex Neuroinflammation Panel 1 Summary
The Simple Plex Human Multianalyte Assay is for the detection of human CHI3L1 and TREM-2 in serum and plasma (EDTA/Heparin).Sample Type & Volume Required Per Well | Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (10 uL) |
Sensitivity | CHI3L1 3.74 pg/mL; TREM-2 0.81 pg/mL |
Assay Range | CHI3L1 6.68-25,500 pg/mL; TREM-2 9.96-15,200 pg/mL |
Product Specifications
Reactivity | Human |
Specificity | Natural and recombinant human CHI3L1 and TREM-2. |
Cross-reactivity | <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested. |
Interference | No significant interference observed with available related molecules. |
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent
Intra-Assay Precision | Inter-Assay Precision | ||||
---|---|---|---|---|---|
Sample | 1 | 2 | 1 | 2 | |
CHI3L1 | n | 16 | 16 | 22 | 22 |
Mean (pg/mL) | 341 | 30,384 | 373 | 26,287 | |
Standard Deviation | 23.5 | 1905 | 35.8 | 2724 | |
CV (%) | 6.9 | 6.3 | 9.6 | 10.4 | |
TREM-2 | n | 16 | 16 | 35 | 37 |
Mean (pg/mL) | 129 | 6668 | 124 | 6144 | |
Standard Deviation | 4.81 | 375 | 12.0 | 619 | |
CV (%) | 3.7 | 5.6 | 9.7 | 10.1 |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % | |
---|---|---|---|
CHI3L1 | CCM (n=2) | 80 | 75-84 |
Specifications
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