Simple Plex Protein A Cartridge

Catalog #: SPCKB-OT-009204 Datasheet / COA / SDS

Simple Plex Protein A assay kit contains cartridge, sample diluent SD62, and wash buffer. Part # starting with ST01 ships in 2-3 days

Catalog #	Availability	Size / Price	Qty
SPCKB-OT-009204
SPCKC-OT-009204
SPCKA-OT-009204
ST01B-OT-009204

Control Products Available

897010: Simple Plex Control for Protein A

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Protein A Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Bioprocess Samples (25 uL)

Sensitivity

4.34 pg/mL

Assay Range

11.5 - 17,500 pg/mL (Bioprocess Samples)

Specificity

Natural and engineered variant Protein A in Bioprocess samples.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of natural and engineered variant Protein A in Bioprocess samples.

Recovery

Recovery at four different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Bioprocess Samples (n=6)	97	90-103

Linearity

Samples containing and/or spiked with high concentrations Protein A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Protein A Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Protein A

Protein A is a Staphylococcus aureus cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially those of the IgG class. Because of this property, it is often utilized for microscopic visualization of molecules and to purify antisera. Protein A has also been used for the treatment of proteinuria in nephrotic syndrome, and severe autoimmune diseases such as rheumatoid arthritis, coeliac disease, and systemic lupus erythematosis.

Entrez Gene IDs:

3919448 (S. aureus)

Alternate Names:

Immunoglobulin G binding protein A; PROA; Protein A; Protein-A; SPA; Staphylococcal protein A