Simple Plex Sepsis Panel
Simple Plex Sepsis Panel Summary
The Simple Plex Human Multianalyte Assay is for the detection of human IL-6 3rd gen, Procalcitonin, TREM-1, uPAR in plasma/serum samples.| Sample Type & Volume Required Per Well | Plasma/Serum (25 uL) |
| Sensitivity | IL-6 3rd gen 0.11 pg/mL; Procalcitonin 0.79 pg/mL; TREM-1 0.73 pg/mL; uPAR 0.2 pg/mL |
| Assay Range | IL-6 3rd gen 0.28 - 2,652 pg/mL; Procalcitonin 1.58 - 15,100 pg/mL; TREM-1 4.2 - 40,000 pg/mL; uPAR 1.68 - 16,000 pg/mL |
Product Specifications
| Reactivity | Human |
| Specificity | Natural and recombinant human IL-6 3rd gen, Procalcitonin, TREM-1, and uPAR. |
| Cross-reactivity | <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules. |
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent
| Intra-Assay Precision | Inter-Assay Precision | ||||
|---|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 | |
| IL-6 3rd gen | n | 16 | 16 | 40 | 40 |
| Mean (pg/mL) | 23.1 | 876/td> | 23.6 | 1008 | |
| Standard Deviation | 0.899 | 72.1 | 1.82 | 77.7 | |
| CV (%) | 3.9 | 8.2 | 7.7 | 7.7 | |
| Procalcitonin | n | 16 | 16 | 18 | 17 |
| Mean (pg/mL) | 81.2 | 4828 | 83.3 | 4876 | |
| Standard Deviation | 5.15 | 301 | 4.69 | 321 | |
| CV (%) | 6.3 | 6.2 | 5.6 | 6.6 | |
| TREM-1 | n | 16 | 16 | 24 | 23 |
| Mean (pg/mL) | 216 | 11,034 | 220 | 11,170 | |
| Standard Deviation | 17.9 | 1013 | 20.1 | 673 | |
| CV (%) | 8.3 | 9.2 | 9.1 | 6.0 | |
| uPAR | n | 16 | 16 | 36 | 35 |
| Mean (pg/mL) | 120 | 6099 | 110 | 5748 | |
| Standard Deviation | 6.8 | 261 | 8.51 | 558 | |
| CV (%) | 5.7 | 4.3 | 7.7 | 9.7 | |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % | |
|---|---|---|---|
| IL-6 3rd gen | EDTA Plasma (n=4) | 92 | 86-102 |
| Heparin Plasma (n=4) | 87 | 85-90 | |
| Serum (n=4) | 94 | 90-99 | |
| Procalcitonin | EDTA Plasma (n=4) | 105 | 97-111 |
| Heparin Plasma (n=4) | 103 | 98-111 | |
| Serum (n=4) | 105 | 96-110 |
Specifications
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