Simple Plex Stocked Panel of IL-15 and IL-7
Simple Plex Stocked Panel of IL-15 and IL-7 Summary
The Simple Plex Human Multianalyte Assay is for the detection of human IL-15 and IL-17 cell culture supernatant samples.Sample Type & Volume Required Per Well | Cell Culture Supernatant (25 uL) |
Sensitivity | IL-15 0.19 pg/mL; IL-17 0.1 pg/mL |
Assay Range | IL-15 0.51 - 1,950 pg/mL; IL-17 1.05 - 10,000 pg/mL |
Product Specifications
Reactivity | Human |
Specificity | Natural and recombinant human IL-15 and IL-17. |
Cross-reactivity | <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested. |
Interference | No significant interference observed with available related molecules. |
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent
Intra-Assay Precision | Inter-Assay Precision | ||||
---|---|---|---|---|---|
Sample | 1 | 2 | 1 | 2 | |
IL-15 | n | 16 | 16 | 10 | 9 |
Mean (pg/mL) | 10.3 | 562 | 10.8 | 540 | |
Standard Deviation | 0.666 | 40.2 | 0.598 | 29.6 | |
CV (%) | 6.5 | 7.2 | 5.6 | 5.5 | |
IL-17 | n | 16 | 16 | 18 | 17 |
Mean (pg/mL) | 57.7 | 2715 | 58.0 | 2513 | |
Standard Deviation | 2.8 | 108 | 3.38 | 188 | |
CV (%) | 4.9 | 4.0 | 5.8 | 7.5 |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % | |
---|---|---|---|
IL-15 | CCS (n=6) | 101 | 94-109 |
Specifications
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