SiRA 2 New
Chemical Name: (5-((2-(2-(2-Aminoethoxy)ethoxy)ethyl)carbamoyl)-2-(7-(dimethylamino)-3-(dimethyliminio)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate)
Purity: ≥98%
Biological Activity
Key information: SiRA 2 (silicon rhodamine 2) is a near-infrared (NIR) fluorogen for SiRA light-up aptamer. SiRA 2 enables imaging of single RNA in living cells.Application: confocal microscopy, super resolution microscopy (SRM) techniques, including super-resolution stimulated emission depletion (STED). Cell permeable.
Properties and Photophysical Data: SiRA 2 is extremely bright, photostable and allows the visualization of mRNA expression in bacteria in no-wash, quencher-free, live-cell imaging experiments. Excitation and emission maxima (λ) are 649 nM and 662 nM, respectively; quantum yield = 0.98; extinction coefficient = 86,000 M-1cm-1; KD = 403 nM.
Reconstitute in 200 μL for a 0.5 mM stock solution. A typical working concentration for this product is 500 nM, see references provided for more details.
We recommend using 5mM Mg2+ (MgCl2 or MgSO4) in all buffers/media for maximum fluorescence intensity.
Optical Data for SiRA 2
| Emission Color | Far Red |
|---|---|
| λabs | 649 nm |
| λem | 662 nm |
| Extinction Coefficient (ε) | 86000 M-1cm-1 |
| Quantum Yield (φ) | 0.98 |
| Closest Laser line | 640 nm |
| Cell Permeable | Yes |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
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