Sleeping Beauty Transposase Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Sleeping Beauty Transposase in Mouse Intestine. Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of intestine from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # MAB2798) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown). Inset panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Sleeping Beauty Transposase in Mouse Liver. Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of liver from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # MAB2798) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown). Inset panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sleeping Beauty Transposase
SB transposase is the catalytic component of a DNA transposition system that catalyzes the excision of the transposon from its original location and promotes its reintegration elsewhere in the genome. An active transposase that acts on transposon sequences found in vertebrate genomes was reconstructed from consensus sequences of several mutated and inactive Tc1/mariner family transposase genes in salmonid fish. The entire 340 amino aa transposase sequence contains a paired-like domain with a leucine zipper for DNA binding, a nuclear localization sequence (NLS), a glycine-rich box and a DD(34)E box. The recognition domain for transposon DNA is contained within the first 123 aa of the transposase (1).
Product Datasheets
Citations for Sleeping Beauty Transposase Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Transposon mutagenesis identifies cooperating genetic drivers during keratinocyte transformation and cutaneous squamous cell carcinoma progression
Authors: A Aiderus, JY Newberg, L Guzman-Roj, AM Contreras-, AL Meshey, DJ Jones, F Amaya-Manz, R Rangel, JM Ward, SC Lee, KH Ban, K Rogers, SM Rogers, L Selvanesan, LA McNoe, NG Copeland, NA Jenkins, KY Tsai, MA Black, KM Mann, MB Mann
PloS Genetics, 2021-08-16;17(8):e1009094.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming
Authors: L Kesselring, C Miskey, C Zuliani, I Querques, V Kapitonov, A Laukó, A Fehér, A Palazzo, T Diem, J Lustig, A Sebe, Y Wang, A Dinnyés, Z Izsvák, O Barabas, Z Ivics
Nucleic Acids Res., 2020-01-10;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A transposon-based analysis reveals RASA1 is involved in triple negative breast cancer
Authors: C Su rez-Cab, RM Quintana, A Bravo, ML Casanova, A Page, JP Alameda, JM Paramio, A Maroto, J Salamanca, AJ Dupuy, A Ram¡rez, M Navarro
Cancer Res, 2017-01-20;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Transposon mutagenesis identifies genes and cellular processes driving epithelial-mesenchymal transition in hepatocellular carcinoma
Authors: Takahiro Kodama
Proc Natl Acad Sci USA, 2016-05-31;113(24):E3384-93.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Sleeping beauty transposition from nonintegrating lentivirus.
Authors: Vink CA, Gaspar HB, Gabriel R, Schmidt M, McIvor RS, Thrasher AJ, Qasim W
Mol. Ther., 2009-05-05;17(7):1197-204.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis
Authors: Donald A Yergeau, Clair M Kelley, Emin Kuliyev, Haiqing Zhu, Michelle R Johnson Hamlet, Amy K Sater et al.
Mobile DNA
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