StemXVivo Cardiomyocyte Maintenance Media Supplement (50X)

A serum-free cell culture media supplement for the extended culture of cardiomyocytes.

Why Culture Cardiomyocytes in a Defined Medium?

Maintaining the health of pluripotent stem cell-derived cardiomyocytes is imperative for generating trustworthy data during downstream experimentation. Culturing cardiomyocytes in serum-free, defined media reduces experimental variability in cell health by eliminating the uncontrolled variables that are often associated with serum-containing media, such as indeterminate levels of vitamins, hormones, and growth factors.

Cardiomyocytes cultured using the Cardiomyocyte Maintenance Media Supplement can also be a part of drug toxicity and small molecule screening workflows where a controlled cell culture environment is essential.

Cardiomyocyte Maintenance Media Use

Human pluripotent stem cells are differentiated into cardiomyocytes using the StemXVivo^® Cardiomyocyte Differentiation Kit (R&D Systems, Catalog # SC032B) until widespread beating is observed in the culture. StemXVivo^® Cardiomyocyte Maintenance Media can then be used to maintain the differentiated cells for extended culture. Media should be replaced daily.

Note: Repeated mechanical stress can negatively affect cell attachment and survival. For extended culture, daily media exchanges should be handled as gently as possible.

Precautions

This product contains human transferrin. This transferrin was purified from donor plasma and tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen.