TFAX 594, SE
Chemical Name: 6-[2-Carboxy-4(5)-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-1,2,10,11-tetrahydro-1,2,2,10,10,11-hexamethyl-4,8-bis(sulfomethyl)pyrano[3,2-g:5,6-g']diquinolin-13-ium inner salt
Biological Activity
TFAX 594, SE is a amine reactive red fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in flow cytometry, 2-photon excitation microscopy, and super resolution microscopy techniques, such as dSTORM. Excitation/emission maxima ~ 590 / 660 nm; extinction coefficient = 92,000 M-1cm-1; quantum yield = 0.66.Optical Data for TFAX 594, SE
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 590 nm |
| λem | 617 nm |
| Extinction Coefficient (ε) | 92,000 M-1cm-1 |
| Quantum Yield (φ) | 0.66 |
| Closest Laser line | 594 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Alternative For | Alexa Fluor® 594, Texas Red®, Bodipy-TR |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates.
Panchuk?Voloshina et al.
J.Histochem.Cytochem., 1999;47:1179
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