TGF-beta 5 Antibody

Catalog #: AB-245-NA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AB-245-NA

TGF‑ beta 5 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 5 Antibody.

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TGF-beta 5 Antibody Summary

Specificity

Detects TGF‑ beta 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with the other TGF-beta variants is observed. Neutralizes the biological activity of recombinant amphibian TGF-beta 5, but not TGF-beta 1, porcine TGF-beta 1.2, TGF-beta 2, or TGF-beta 3.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant amphibian TGF-beta 5
Gly271-Ser382
Accession # P16176

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Amphibian TGF-beta 5 (Catalog # 245-B5)

Neutralization

Measured by its ability to neutralize TGF‑ beta 5 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 0.03-0.08 µg/mL in the presence of 0.1 ng/mL Recombinant Amphibian TGF‑ beta 5 and 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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TGF‑ beta 5 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 5 Antibody. Recombinant Amphibian TGF-beta 5 (Catalog # 245-B5) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Amphibian TGF-beta 5 (0.1 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-TGF-beta 5 Polyclonal Antibody (Catalog # AB-245-NA). The ND₅₀ is typically 0.03-0.08 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 5

TGF-beta 5 is a member of the TGF-beta family of growth factors. These proteins are stable, multifunctional factors with a wide variety of effects on the growth and differentiation of virtually all cell types. These actions on growth or differentiation may be stimulatory or inhibitory, depending on the cell type, growth conditions, state of differentiation and on the presence of other growth factors. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. The in vivo role of TGF-beta 5 is also not known, but the presence of this factor in Xenopus embryos, tadpole cells, and adult tissues suggests a role in the mediation of differentiation and development. Similar roles have been suggested for other members of the TGF-beta family. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

Long Name

Transforming Growth Factor beta 5

Entrez Gene IDs

397778 (Amphibian)

Alternate Names

CED;DPD1;IBDIMDE;LAP;TGFB;TGFB1;TGFbeta;TGF-beta1;Transforming growth factor beta;Transforming growth factor beta-1 proprotein; TGFbeta 5; TGF-beta 5