trans-Ned 19
Chemical Name: (1R,3S)-1-[3-[[4-(2-Fluorophenyl)piperazin-1-yl]methyl]-4-methoxyphenyl]-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid
Purity: ≥98%
Biological Activity
trans-Ned 19 is a nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist. Inhibits NAADP-mediated Ca2+ release (IC50 = 6 nM); also inhibits [32P]NAADP binding (IC50 = 0.4 nM). Fluorescently labels NAADP receptors in intact cells. Stereosiomer of cis-Ned 19 (Cat. No. 3953).Optical Data for trans-Ned 19
| λabs | 368 nm |
|---|---|
| λem | 425 nm |
| Closest Laser line | 355 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
-
Arachidonic acid triggers [Ca2+]i increases in rat round spermatids by a likely GPR activation, ERK signalling and ER/acidic compartments Ca2+ release
J Paillamanq, A Sanchez-Tu, EM Carmona, CL Treviño, C Sandoval, F Nualart, N Osses, JG Reyes
PLoS ONE, 2017;12(2):e0172128. -
Identification of a chemical probe for NAADP by virtual screening.
Naylor et al.
Nat.Chem.Biol., 2009;5:220 -
Analogues of the nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist Ned-19 indicate two binding sites on the NAADP receptor.
Rosen et al.
J.Biol.Chem., 2009;284:34930 -
NAADP receptors mediate calcium signaling stimulated by endothelin-1 and NE in renal afferent arterioles.
Thai et al.
Am.J.Physiol.Renal Physiol., 2009;297:F510
Product Datasheets
Reconstitution Calculator
Molarity Calculator
Citations for trans-Ned 19
The citations listed below are publications that use Tocris products. Selected citations for trans-Ned 19 include:
6 Citations: Showing 1 - 6
-
Mu opioid receptor-mediated release of endolysosome iron increases levels of mitochondrial iron, reactive oxygen species, and cell death.
Authors: Olimpia Et al.
NeuroImmune Pharm Ther 2023;2:19-35
-
TPC2 promotes choroidal angiogenesis and inflammation in a mouse model of neovascular age-related macular degeneration.
Authors: Zhuo Et al.
Life Sci Alliance 2021;4
-
Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis.
Authors: Simon P Et al.
Elife 2021;10
-
Cross-talk between CD38 and TTP is essential for resolution of inflammation during microbial sepsis.
Authors: Joe Et al.
Cell Rep. 2020;30:1063
-
Lysosomal Fusion: An Efficient Mechanism Increasing Their Sequestration Capacity for Weak Base Drugs without Apparent Lysosomal Biogenesis.
Authors: Petr Et al.
Biomolecules 2020;10
-
NAADP-Dependent Ca(2+) Signaling Controls Melanoma Progression, Metastatic Dissemination and Neoangiogenesis.
Authors: Favia Et al.
Toxicol Lett 2016;6:18925
FAQs
No product specific FAQs exist for this product, however you may
View all Small Molecule FAQsReviews for trans-Ned 19
There are currently no reviews for this product. Be the first to review trans-Ned 19 and earn rewards!
Have you used trans-Ned 19?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
