V5 Epitope Tag Antibody Summary
Accession # Y138513
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of V5 Epitope-tagged proteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged CBL, N-terminal V5-tagged FSNC4, C-terminal V5-tagged LAMP2B, and C-terminal V5-tagged GALNT2. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for V5 Epitope-tagged at approximately 110 kDa, 34 kDa, 75 kDa, and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of V5 Epitope Tag in HEK293 Human Cell Line Transfected with V5-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with V5-tagged proteins was stained with Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
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Detection of V5 Epitope Tag by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged CBL and C-terminal V5-tagged GALNT2, loaded at 0.2 mg/mL. Specific bands were detected for V5 Epitope-tagged proteins at approximately 119 kDa and 65 kDa (as indicated) using 1 µg/mL of Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Mouse V5 Epitope Tag Antibody by Immunoprecipitation Identification of DCHS1-LIX1L-SEPT9 (DLS) protein complex. (A) Yeast two-hybrid (Y2H) screen using cytoplasmic tail (A.A. 2962–3191) of Dachsous Cadherin Related-1 (DCHS1) as bait reveals Lix1-Like (LIX1L). (B) Y2H with full-length LIX1-Like (LIX1L) identifies DCHS1 and septin-9 (SEPT9) as binding partners. In A and B, confidence scores represent likelihood of an interaction, with A being the highest level of confidence (See Methods for details). (C) Diagram depicting interacting proteins and binding domains. (D) Co-Immunoprecipitation (co-IP) and immunoblotting (IB) analysis with DCHS1-V5 and LIX1L-FLAG transfected in HEK293T cells confirms protein interaction. (E) Co-IP and IB of cVIC protein lysate treated with 10 μg peptide mimicking the LIX1L-SEPT9 binding domain (S9-FWD:5-FAM-YGRKKRRQRRR-Ahx TKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDIT-Lys(Biotin), S9-REV:5-FAM-YGRKKRRQRRR-Ahx-TIDKINQMHTRILLDRLYAFECHTTNEVEITGWKT-Lys(Biotin)). (F) IB of DCHS1-V5, LIX1L-FLAG and SEPT9-HA co-transfected into HEK293T cells depicts stabilization of DCHS1 protein only in the presence of LIX1L. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35200715), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse V5 Epitope Tag Antibody by Immunoprecipitation Identification of DCHS1-LIX1L-SEPT9 (DLS) protein complex. (A) Yeast two-hybrid (Y2H) screen using cytoplasmic tail (A.A. 2962–3191) of Dachsous Cadherin Related-1 (DCHS1) as bait reveals Lix1-Like (LIX1L). (B) Y2H with full-length LIX1-Like (LIX1L) identifies DCHS1 and septin-9 (SEPT9) as binding partners. In A and B, confidence scores represent likelihood of an interaction, with A being the highest level of confidence (See Methods for details). (C) Diagram depicting interacting proteins and binding domains. (D) Co-Immunoprecipitation (co-IP) and immunoblotting (IB) analysis with DCHS1-V5 and LIX1L-FLAG transfected in HEK293T cells confirms protein interaction. (E) Co-IP and IB of cVIC protein lysate treated with 10 μg peptide mimicking the LIX1L-SEPT9 binding domain (S9-FWD:5-FAM-YGRKKRRQRRR-Ahx TKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDIT-Lys(Biotin), S9-REV:5-FAM-YGRKKRRQRRR-Ahx-TIDKINQMHTRILLDRLYAFECHTTNEVEITGWKT-Lys(Biotin)). (F) IB of DCHS1-V5, LIX1L-FLAG and SEPT9-HA co-transfected into HEK293T cells depicts stabilization of DCHS1 protein only in the presence of LIX1L. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35200715), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: V5 Epitope Tag
Product Datasheets
Citations for V5 Epitope Tag Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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The ARID1A-METTL3-m6A axis ensures effective RNase H1-mediated resolution of R-loops and genome stability
Authors: Zhang, J;Chen, F;Tang, M;Xu, W;Tian, Y;Liu, Z;Shu, Y;Yang, H;Zhu, Q;Lu, X;Peng, B;Liu, X;Xu, X;Gullerova, M;Zhu, WG;
Cell reports
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
DCHS1, Lix1L, and the Septin Cytoskeleton: Molecular and Developmental Etiology of Mitral Valve Prolapse
Authors: KS Moore, R Moore, DB Fulmer, L Guo, C Gensemer, R Stairley, J Glover, TC Beck, JE Morningsta, R Biggs, R Muhkerjee, A Awgulewits, RA Norris
Journal of cardiovascular development and disease, 2022-02-17;9(2):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Host and viral determinants for efficient SARS-CoV-2 infection of the human lung
Authors: H Chu, B Hu, X Huang, Y Chai, D Zhou, Y Wang, H Shuai, D Yang, Y Hou, X Zhang, TT Yuen, JP Cai, AJ Zhang, J Zhou, S Yuan, KK To, IH Chan, KY Sit, DC Foo, IY Wong, AT Ng, TT Cheung, SY Law, WK Au, MA Brindley, Z Chen, KH Kok, JF Chan, KY Yuen
Nature Communications, 2021-01-08;12(1):134.
Species: Hamster - Mesocricetus auratus (Golden Hamster)
Sample Types: Cell Lysates
Applications: Western Blot -
Forward genetics identifies a novel sleep mutant with sleep state inertia and REM sleep deficits
Authors: GT Banks, MCC Guillaumin, I Heise, P Lau, M Yin, N Bourbia, C Aguilar, MR Bowl, C Esapa, LA Brown, S Hasan, E Tagliatti, E Nicholson, RS Bains, S Wells, VV Vyazovskiy, K Volynski, SN Peirson, PM Nolan
Sci Adv, 2020-08-12;6(33):eabb3567.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
The Zika Virus Capsid Disrupts Corticogenesis by Suppressing Dicer Activity and miRNA Biogenesis
Authors: J Zeng, S Dong, Z Luo, X Xie, B Fu, P Li, C Liu, X Yang, Y Chen, X Wang, Z Liu, J Wu, Y Yan, F Wang, JF Chen, J Zhang, G Long, SA Goldman, S Li, Z Zhao, Q Liang
Cell Stem Cell, 2020-08-06;0(0):.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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