Viral B18R Antibody

Catalog #: AF8185 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8185
AF8185-SP

Detection of Viral B18R by Western Blot.

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Viral B18R Antibody Summary

Species Reactivity

Viral

Specificity

Detects viral B18R in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant viral B18R
His20-Glu351
Accession # P25213

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Viral B18R by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with viral B18R. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Viral B18R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for B18R at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B18R

B18R (Soluble interferon alpha/beta receptor B18) is a 60-65 kDa protein encoded by the Vaccinia virus genome and by the genomes of other orthopoxviruses. Its function represents one of several mechanisms used by these viruses to evade the host immune response (1, 2). It is known as B18R in the Western Reserve (WR) strain of Vaccinia but as B19R in the Copenhagen strain (3). There is a structurally-unrelated, larger Vaccinia protein that is also known as B18R (or B16R) that contains multiple ankyrin-like repeats (4). The soluble interferon receptor B18R, however, contains three immunoglobulin-like domains and shows homology to human, mouse, and bovine type I interferon receptors (5). The Wyeth strain of Vaccinia virus encodes a truncated protein that lacks the C-terminal Ig-like domain, and B18R is functionally absent in the Lister strain (6, 7). B18R functions as a decoy receptor for type I interferons (IFN alpha, beta, omega). It binds to type I interferons from multiple species and prevents IFN signaling through its receptors (6-8). B18R binds to the surface of virus infected and uninfected cells where it retains its capacity to bind and neutralize IFN (6, 8). It shields those cells from the antiviral effects of type I interferons, thereby enabling virus replication and pathogenicity (6-8). B18R also limits the effectiveness of IFN alpha produced following TLR activation (9), and it limits adaptive T cell responses (3). B18R is a common factor used for increasing the efficiency of RNA reprogramming in induced pluripotent stem cells (IPS cells) (10).

References