Viral Tick-borne Encephalitis Virus NS1 Antibody

Catalog # Availability Size / Price Qty
MAB10106-100
MAB10106-SP
Viral Tick-borne Encephalitis Virus NS1 ELISA Standard Curve.
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Product Details
Citations (1)
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Viral Tick-borne Encephalitis Virus NS1 Antibody Summary

Species Reactivity
Viral
Specificity
Detects Tick-borne Encephalitis Virus NS1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1004134
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived Tick-borne Encephalitis Virus NS1
Amino Acids 777-1128
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral Tick-borne Encephalitis Virus NS1 Monoclonal Antibody (Catalog # MAB101061).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Viral Tick-borne Encephalitis Virus NS1 ELISA Standard Curve. Recombinant Viral Tick-borne Encephalitis Virus NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Tick-borne Encephalitis Virus NS1 Monoclonal Antibody (Catalog # MAB101061) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Tick-borne Encephalitis Virus NS1 Monoclonal Antibody (Catalog # MAB10106) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tick-borne Encephalitis Virus NS1

Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus, is the virus associated with tick-borne encephalitis. Three TBEV sub-types have been described: European or Western tick-borne encephalitis virus, Siberian tick-borne encephalitis virus, and Far eastern Tick-borne encephalitis virus (formerly known as Russian Spring Summer encephalitis virus, RSSEV). TEBV NS1 protein is one of the most conserved. Homology level of the NS1 protein is about 90% between TBEV strains. Tick-borne encephalitis virus causes 13,000 cases of human meningitis and encephalitis annually.

Entrez Gene IDs
1489719 (Viral)
Alternate Names
TBEV NS1; TBEV-NS1; Tick-borne Encephalitis Virus NS1

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Citation for Viral Tick-borne Encephalitis Virus NS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Safety and immunogenicity following co-administration of Yellow fever vaccine with Tick-borne encephalitis or Japanese encephalitis vaccines: Results from an open label, non-randomized clinical trial
    Authors: JT Sandberg, M Löfling, R Varnait?, J Emgård, N Al-Tawil, L Lindquist, S Gredmark-R, J Klingström, K Loré, K Blom, HG Ljunggren
    PloS Neglected Tropical Diseases, 2023-02-09;17(2):e0010616.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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