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Viral wnvNS3 Protease Antibody

Catalog #: MAB29071
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB29071
MAB29071-SP
Detection of wnvNS3 Protease by Western Blot.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral wnvNS3 Protease Antibody Summary

Species Reactivity
Viral
Specificity
Detects West Nile NS3 Protease in Western blots.
Source
Monoclonal Mouse IgG3 Clone # 345712
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant wnvNS3 Protease protein
Ser1423-Lys1470, Gly1506-Leu1689
Accession # ABD85079
Formulation
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Viral wnvNS3 Protease (0.5 µg/mL, Catalog # 2907-SE) cleavage of the fluorogenic peptide substrate pERTKR-Amc (20 µM, Catalog # ES013). The Neutralization Dose (ND50) is typically 15 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of wnvNS3 Protease antibody by Western Blot. View Larger

Detection of wnvNS3 Protease by Western Blot. Western blot shows lysates of Recombinant Viral wnvNS3 Protease. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa and C-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile Tris-buffered saline, pH 7.3.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: wnvNS3 Protease

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein used as an immunogen consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with a G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.

References
  1. Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
  2. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.
Long Name
West Nile Virus NS3 Protease
Entrez Gene IDs
912267 (Viral)
Alternate Names
wnvNS3 Protease

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Citations for Viral wnvNS3 Protease Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. P-body components LSM1, GW182, DDX3, DDX6 and XRN1 are recruited to WNV replication sites and positively regulate viral replication
    Authors: Harendra S. Chahar, Shuiping Chen, N. Manjunath
    Virology
  2. West Nile virus infection causes endocytosis of a specific subset of tight junction membrane proteins.
    Authors: Xu Z, Waeckerlin R, Urbanowski MD
    PLoS ONE, 2012-05-24;7(5):e37886.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. The capsid-binding nucleolar helicase DDX56 is important for infectivity of West Nile virus.
    Authors: Xu Z, Anderson R, Hobman TC
    J. Virol., 2011-03-16;85(11):5571-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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