Viral wnvNS3 Protease Antibody

Catalog #: MAB2907
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2907
MAB2907-SP

Detection of wnvNS3 Protease by Western Blot.

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Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral wnvNS3 Protease Antibody Summary

Species Reactivity

Viral

Specificity

Detects viral wnvNS3 Protease in direct ELISAs and Western blots. Recognizes an epitope in the NS3 domain.

Source

Monoclonal Mouse IgG₁ Clone # 345713

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant West Nile Virus NS3 Protease, expressed as a fusion protein with co-factor NS2L
Gly1506-Leu1689
Accession # ABD85079

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of wnvNS3 Protease by Western Blot. Western blot shows Recombinant Viral wnvNS3 Protease (Catalog # 2907-SE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB2907) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa and and C-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: wnvNS3 Protease

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the co‑factor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with the G₄SG₄ linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.

References

Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.

Long Name

West Nile Virus NS3 Protease

Entrez Gene IDs

912267 (Viral)

Alternate Names

wnvNS3 Protease

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Viral wnvNS3 Protease Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Exposure of negative-sense viral RNA in the cytoplasm initiates innate immunity to West Nile virus
Authors: Genoyer, E;Wilson, J;Ames, JM;Stokes, C;Moreno, D;Etzyon, N;Oberst, A;Gale, M;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry
The capsid-binding nucleolar helicase DDX56 is important for infectivity of West Nile virus.
Authors: Xu Z, Anderson R, Hobman TC
J. Virol., 2011-03-16;85(11):5571-80.
Species: Human
Sample Types: Whole Cells
Applications: ICC