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VisUBlock Mouse on Mouse Blocking Reagent

Catalog #: VB001
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
VB001-01ML
Product Details
Citations (1)
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VisUBlock Mouse on Mouse Blocking Reagent Summary

Kit Summary

Blocking reagent for use with primary antibodies from mouse host and mouse tissue.

Key Benefits

  • Reduces background staining
  • Enables staining of mouse tissue with mouse IgG primary antibodies
 

 

Reagents Provided

1mL VisUBlock™ Mouse on Mouse Blocking Reagent

Stability and Storage

Store at 2 °C to 8 °C. DO NOT FREEZE.

Precaution

See MSDS.

Reagent Preparation

Mix 30 µL of VisUBlock™ Mouse on Mouse Blocking Reagent with 1mL of preferred buffer such as PBS or TBS. This will produce approximately 30 mL of working solution, which will stain 100-250 tissue sections.

Procedure

  1. Prior to primary antibody incubation, apply Mouse on Mouse IgG Blocking Reagent to tissue section. Incubate 1 hour at room temperature.
  2. Tap off, do not rinse, and continue with primary antibody incubation and detection antibody incubation according to protocol set in lab.

Limitations

  • FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
  • Results may vary due to variations in tissue staining conditions.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
Control
Species
Mouse

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Citation for VisUBlock Mouse on Mouse Blocking Reagent

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Immunohistochemical Detection of Estrogen Receptor-Beta (ERbeta) with PPZ0506 Antibody in Murine Tissue: From Pitfalls to Optimization
    Authors: SK Schröder, CG Tag, JC Kessel, P Antonson, R Weiskirche
    Biomedicines, 2022-12-01;10(12):.  2022-12-01

FAQs

  1. Is VisUBlock Mouse on Mouse Blocking Reagent, catalog # VB001, compatible with an anti-mouse secondary antibody raised in Donkey?

    • Yes, VisUBlock Blocking Reagent is compatible with anti-mouse secondary antibodies raised in any species.

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