Vitamin D3 Antibody

Catalog #: MAB6566 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6566
MAB6566-SP

Detection of Vitamin D3 in Human monocyte-derived dendritic cells by Flow Cytometry.

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All Vitamin D3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Vitamin D3 Antibody Summary

Specificity

Detects Vitamin D3 in flow cytometry.

Source

Monoclonal Mouse IgG₁ Clone # 685503

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

25-Hydroxyvitamin D₃

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Vitamin D3 antibody in Human monocyte-derived dendritic cells antibody by Flow Cytometry.

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Detection of Vitamin D3 in Human monocyte-derived dendritic cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Vitamin D3 Monoclonal Antibody (Catalog # MAB6566, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitamin D3

25-hydroxy-vitamin D₃ (25(OH)D₃) is synthesized in the liver and is the primary circulating form of vitamin D. Its blood concentration, which reflects 25(OH)D₃ produced by exposure to ultraviolet B, as well as dietary and vitamin D supplementation, is felt to be the best indicator of vitamin D status. 25(OH)D₃ is metabolized to 1 alpha,25(OH)₂D₃ in the proximal tubular cells of the kidney by the enzyme 1 alpha -hydroxylase. The vitamin D structure is similar to that of classic steroid hormones, such as estradiol, cortisol, and aldosterone in that they have the same root cyclopentanoperhydrophenanthrene ring structure. It has been shown that the active steroid hormone 1 alpha,25(OH)₂D₃ is essential for life in higher animals. Besides playing important roles in calcium homeostasis and bone mineral metabolism, it is now known to play a role in cellular differentiation, inhibition of cell growth, immune regulation and the prevention of neoplastic transformation. The active form of vitamin D₃, 1 alpha,25(OH)₂D₃, acts both through its cellular receptor, the vitamin D receptor (VDR), and through other extrarenal targets in an autocrine and paracrine manner where 1 alpha -hydroxylase is present.