IL-2 Receptor alpha in Mouse Spleen.
Interleukin-2 Receptor alpha (IL-2 R alpha) was detected in immersion fixed frozen sections of mouse spleen using Mouse IL-2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2438) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
This antibody specifically recognizes mouse IL-2 Receptor alpha. In ELISA and Western blot experiments, this antibody shows approximately 5% cross-reactivity with recombinant human IL-2 R alpha and less than 1% cross-reactivity with recombinant mouse IL-2 R beta and IL-2 R gamma.
Secondary Antibodies
R&D Systems offers a wide range of biotinylated, HRP-conjugated, fluorochrome-labeled, and unlabeled species-specific secondary antibodies. Our NorthernLights™ fluorescent secondary antibodies are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.