Your Guide to Immunohistochemistry (IHC) Techniques and Protocols
Immunohistochemistry (IHC) is a commonly used technique for detecting the expression of one or more antigens in a fixed tissue section. The most challenging aspect of this technique is determining and optimizing the experimental conditions required to generate a strong and specific signal for each antigen of interest. Based on information provided by scientists at R&D Systems IHC/ICC core facility, this guide is intended to serve as a reference for designing, performing, optimizing, and troubleshooting IHC protocols.
Download this handbook to explore:
- Techniques for designing a successful IHC experiment
- Easy-to-follow protocols for chromogenic and fluorescent staining of antigens in tissue sections
- RNA-protein co-detection in the same tissue sample using RNA in situ hybridization (ISH) and IHC
- Multiplexing IHC
- IHC Controls
- Troubleshooting suggestions that will help solve a range of IHC-related issues
