"Isotype Controls" has 45 results in Resources.

Appropriate controls are critical for the accurate interpretation of IHC/ICC results. A satisfactory IHC/ICC experimental design produces results that demonstrate that the antigen is localized to the correct specialized tissues, cell types, or subcellular location. Optimization of fixation, blocking, antibody incubation, and antigen retrieval steps will generate a strong and specific signal....

Peridinin Chlorophyll Protein Complex (PerCP)-conjugated antibod­ies are convenient tools for use in flow cytometry experiments. PerCP is a water soluble carotenoid pigment found in photosynthetic dino­flagellates. It is excited by a 488 nm argon laser, and with a relatively large Stokes shift, emits at a maximum wavelength of 675 nm. Be­cause of these spectral characteristics, there is...

Figure 1. The use of the isotype control alone (yellow) may lead to an overestimation of the number of cells staining positive for intracellular IL-1 beta. The different staining patterns between permeabilized (red) and non-permeabilized (blue) cells represents intracellular cytokine staining. Method: Peripheral blood (PMN) cells were stained for IL-1 beta after culture with 0.5 µg/mL...

Complete Listing of Multi-Color Flow Cytometry Kits Flow cytometry kits designed for analysis of cell populations use up to four fluorochrome-conjugated antibodies. Product Summary Flow cytometry is a powerful tool that is capable of rapidly measuring several cellular parameters simultaneously. Because each cell is assessed individually, the technique excels in experiments designed to...

Antibody Isotype Controls A wide selection of species-specific IgG isotype controls conjugated to a variety of labels. Secondary Antibodies We offer a range of secondary antibodies for flow cytometry, immunohistochemistry, and Western blotting. Staining Reagents for Flow Cytometry Flow Cytometry Staining Buffer (1X) (Catalog # FC001) For immunofluorescent staining of single-cell...

To provide further customer support, we have posted answers to the most frequently asked questions about our products. Product Category Antibodies Antibody Isotype Controls Apoptosis Detection Kits & Reagents Cell Culture Products ELISAs ELISpot/FluoroSpot Kits & Development Modules Enzyme Activity Assays & Reagents Flow Cytometry Kits Luminex Assays Primer...

Flow Cytometry Staining Overview Immunophenotyping suspended cells based on antigens present on the cell surface is one of the most common uses of flow cytometry. Since cell surface proteins are readily accessible to the antibody, a permeabilization step is not required. By staining cell surface markers, researchers can identify specific cell populations and perform fluorescence-activated...

CCR7 CCR7, an orphan receptor formerly known as EBI1 (EBV-induced gene 1),1 is the chemokine receptor for CCL19/ELC/MIP-3 beta and CCL21/SLC/6Ckine.2-4 It is expressed on T cells and dendritic cells (DC), consistent with the chemotactic action of CCL19 and CCL21 for both lymphocytes and mature DC. Both memory (CD45RO+) and naïve (CD45RA+) CD4+ and CD8+ T cells express the CCR7 receptor.5 Within...

Because of their complexity, some of the initial work on chemokine receptors has been performed with polyclonal antibodies derived from peptide immunogens. While these anti-peptide reagents are often useful in the detection of denatured proteins on western blots, they have limited usefulness in the detection of membrane-anchored native proteins. R&D Systems now uses an alternative approach to...

For the simultaneous detection of mesenchymal stem cell markers by flow cytometry. Product Summary The Human Mesenchymal Stromal Cell Multi-Color Flow Cytometry Kit (Catalog # FMC002) and the Mouse Mesenchymal Stromal Cell Multi-Color Flow Cytometry Kit (Catalog # FMC003) each contain four fluorochrome-conjugated monoclonal antibodies and corresponding isotype controls that can be used for...