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Normal Sheep IgG Control

Catalog #: 5-001-A
42 Citations 6 Reviews Datasheet / COA / SDS
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5-001-A
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Detection of Normal Sheep IgG Control by Control
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Citations (42)
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Reviews (6)
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Normal Sheep IgG Control Summary

Source
Polyclonal Sheep IgG
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Normal Sheep IgG Control by Control View Larger

Detection of Normal Sheep IgG Control by Control Effects of SDF-1-CXCR4/CXCR7 pathway on MSC chemotaxis in vitro.(A) The chemotaxis in response to SDF-1 alpha (10 ng/ml for 12 h) was performed in the NP-MSCs and HP-MSCs treated with a neutralizing anti-CXCR4 antibody, an anti-CXCR7 antibody, and the respective isotype-matched control antibodies. *P<0.05, vs NP-MSCs; †P<0.05, vs the respective isotype-matched control antibodies. (B) NP-MSCs were transiently overexpressed with CXCR4 using pORF9-mCXCR4 vector or with CXCR7 using pORF9-mCXCR7 vector (n = 6). A negative control empty (pORF9-MCS) vector was used. (C) The transfected cells were subjected to chemotaxis in response to the indicated concentrations of SDF-1 alpha for 12 h. *P<0.05, vs the empty vector. (D and E) Western blot analysis (D) and FCM (E) were performed to determine the intracellular and extracellular expression of both CXCR4 and CXCR7 in the cells treated with or without SDF-1 alpha (50 ng/ml for 60 min). (F) The chemotaxis in response to SDF-1 alpha was performed in the cells treated with or without SDF-1 alpha. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22511954), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Normal Sheep IgG Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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  1. Stiffness and axial pain are associated with the progression of calcification in a mouse model of diffuse idiopathic skeletal hyperostosis
    Authors: DE Fournier, MA Veras, CR Brooks, D Quinonez, M Millecamps, LS Stone, CA Séguin
    Arthritis Research & Therapy, 2023-04-29;25(1):72.
  2. Alzheimer's disease protective allele of Clusterin modulates neuronal excitability through lipid-droplet-mediated neuron-glia communication
    Authors: Zhao, X;Li, Y;Zhang, S;Sudwarts, A;Zhang, H;Kozlova, A;Moulton, MJ;Goodman, LD;Pang, ZP;Sanders, AR;Bellen, HJ;Thinakaran, G;Duan, J;
    medRxiv : the preprint server for health sciences
    Species: Human, Mouse
    Sample Types: Chromatin
    Applications: Chromatin Immunoprecipitation (Negative)
  3. Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal Cancer
    Authors: VA Stark, COB Facey, L Opdenaker, JZ Fields, BM Boman
    bioRxiv : the preprint server for biology, 2023-02-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The BNT162b2 mRNA SARS-CoV-2 vaccine induces transient afucosylated IgG1 in naive but not in antigen-experienced vaccinees
    Authors: J Van Coilli, T Pongracz, J Rahmöller, HJ Chen, CE Geyer, LA van Vught, JS Buhre, T Šušti?, TLJ van Osch, M Steenhuis, W Hoepel, W Wang, AS Lixenfeld, J Nouta, S Keijzer, F Linty, R Visser, MD Larsen, EL Martin, I Künsting, S Lehrian, V von Kopylo, C Kern, HB Lunding, M de Winther, N van Mourik, T Rispens, T Graf, MA Slim, RP Minnaar, MK Bomers, JJ Sikkens, APJ Vlaar, CE van der Sc, J den Dunnen, M Wuhrer, M Ehlers, G Vidarsson, Fatebenefr, UMC COVID-
    EBioMedicine, 2022-12-16;87(0):104408.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  5. Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication
    Authors: Marco Gargaro, Giulia Scalisi, Giorgia Manni, Carlos G. Briseño, Prachi Bagadia, Vivek Durai et al.
    Immunity
  6. Development and Preliminary Testing of Porcine Blood-Derived Endothelial-like Cells for Vascular Tissue Engineering Applications: Protocol Optimisation and Seeding of Decellularised Human Saphenous Veins
    Authors: Andrew Bond, Vito Bruno, Jason Johnson, Sarah George, Raimondo Ascione
    International Journal of Molecular Sciences
  7. Fringe GlcNAc-transferases differentially extend O-fucose on endogenous NOTCH1 in mouse activated T cells
    Authors: K Matsumoto, V Kumar, S Varshney, AV Nairn, A Ito, F Pennarubia, KW Moremen, P Stanley, RS Haltiwange
    The Journal of Biological Chemistry, 2022-05-25;0(0):102064.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  8. Environmental cues from neural crest derivatives act as metastatic triggers in an embryonic neuroblastoma model
    Authors: D Ben Amar, K Thoinet, B Villalard, O Imbaud, C Costechare, L Jarrosson, F Reynaud, J Novion Duc, Y Couté, JF Brunet, V Combaret, N Corradini, C Delloye-Bo, V Castellani
    Nature Communications, 2022-05-10;13(1):2549.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  9. Structural basis for HCMV Pentamer receptor recognition and antibody neutralization
    Authors: M Kschonsak, MC Johnson, R Schelling, EM Green, L Rougé, H Ho, N Patel, C Kilic, E Kraft, CP Arthur, AL Rohou, L Comps-Agra, N Martinez-M, L Perez, J Payandeh, C Ciferri
    Science Advances, 2022-03-11;8(10):eabm2536.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  10. Plasmon-enhanced Quantitative Lateral Flow Assay for Femtomolar Detection of SARS-CoV-2 Antibodies and Antigens
    Authors: R Gupta, P Gupta, Z Wang, A Seth, J Morrissey, I George, S Gandra, G Storch, B Parikh, G Genin, S Singamanen
    Research square, 2022-02-21;0(0):.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  11. Adipose-Derived Stem Cells from Type 2 Diabetic Rats Retain Positive Effects in a Rat Model of Erectile Dysfunction
    Authors: Marlene Louise Quaade, Pratibha Dhumale, Simon Gabriel Comerma Steffensen, Hans Christian Beck, Eva Bang Harvald, Charlotte Harken Jensen et al.
    International Journal of Molecular Sciences
  12. Roles of OX40 and OX40 Ligand in Mycosis Fungoides and S�zary Syndrome
    Authors: Y Kawana, H Suga, H Kamijo, T Miyagaki, M Sugaya, S Sato
    International Journal of Molecular Sciences, 2021-11-22;22(22):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization Control
  13. Z-form extracellular DNA is a structural component of the bacterial biofilm matrix
    Authors: John R. Buzzo, Aishwarya Devaraj, Erin S. Gloag, Joseph A. Jurcisek, Frank Robledo-Avila, Theresa Kesler et al.
    Cell
  14. Differential Marker Expression between Keratinocyte Stem Cells and Their Progeny Generated from a Single Colony
    Authors: D Ali, D Alhattab, H Jafar, M Alzubide, N Sharar, S Bdour, A Awidi
    International Journal of Molecular Sciences, 2021-10-06;22(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Differential plasmacytoid dendritic cell phenotype and type I Interferon response in asymptomatic and severe COVID-19 infection
    Authors: M Severa, RA Diotti, MP Etna, F Rizzo, S Fiore, D Ricci, M Iannetta, A Sinigaglia, A Lodi, N Mancini, E Criscuolo, M Clementi, M Andreoni, S Balducci, L Barzon, P Stefanelli, N Clementi, EM Coccia
    PloS Pathogens, 2021-09-02;17(9):e1009878.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization Control
  16. Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling
    Authors: Ross A. Robinson, Samuel C. Griffiths, Lieke L. van de Haar, Tomas Malinauskas, Eljo Y. van Battum, Pavol Zelina et al.
    Cell
  17. CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth
    Authors: X Zhuang, F Maione, J Robinson, M Bentley, B Kaul, K Whitworth, N Jumbu, E Jinks, J Bystrom, P Gabriele, E Garibaldi, E Delmastro, Z Nagy, D Gilham, E Giraudo, R Bicknell, SP Lee
    JCI Insight, 2020-10-02;5(19):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Control
  18. Cancer-associated fibroblasts downregulate type I interferon receptor to stimulate intratumoral stromagenesis
    Authors: C Cho, R Mukherjee, AR Peck, Y Sun, N McBrearty, KV Katlinski, J Gui, PK Govindaraj, E Puré, H Rui, SY Fuchs
    Oncogene, 2020-08-17;0(0):.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  19. Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia
    Authors: CJ Mietus, TJ Lackner, PS Karvelis, GT Willcockso, CM Shields, NG Lambert, P Koutakis, MA Fuglestad, H Hernandez, GR Haynatzki, JKS Kim, HK DeSpiegela, II Pipinos, GP Casale
    J Clin Med, 2020-08-08;9(8):.
    Species: Bovine, Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  20. An evaluation of the tumour endothelial marker CLEC14A as a therapeutic target in solid tumours
    Authors: J Robinson, K Whitworth, E Jinks, Z Nagy, R Bicknell, SP Lee
    J Pathol Clin Res, 2020-07-21;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  21. Phosphatidylserine on viable sperm and phagocytic machinery in oocytes regulate mammalian fertilization
    Authors: CM Rival, W Xu, LS Shankman, S Morioka, S Arandjelov, CS Lee, KM Wheeler, RP Smith, LB Haney, BE Isakson, S Purcell, JJ Lysiak, KS Ravichandr
    Nat Commun, 2019-10-01;10(1):4456.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation
    Authors: T Kuroda, S Yasuda, S Tachi, S Matsuyama, S Kusakawa, K Tano, T Miura, A Matsuyama, Y Sato
    Nat Commun, 2019-05-15;10(1):2175.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  23. Sestrin modulator NV-5138 produces rapid antidepressant effects via direct mTORC1 activation
    Authors: T Kato, S Pothula, RJ Liu, CH Duman, R Terwillige, GP Vlasuk, E Saiah, S Hahm, RS Duman
    J. Clin. Invest., 2019-04-16;130(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  24. iRGD synergizes with PD-1 knockout immunotherapy by enhancing lymphocyte infiltration in gastric cancer
    Authors: N Ding, Z Zou, H Sha, S Su, H Qian, F Meng, F Chen, S Du, S Zhou, H Chen, L Zhang, J Yang, J Wei, B Liu
    Nat Commun, 2019-03-22;10(1):1336.
    Species: Human
    Sample Types: Spheroids
    Applications: Neutralization
  25. Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma
    Authors: JH Wang, ST Huang, L Zhang, ZG Liu, RX Liang, SW Jiang, YN Jiang, XJ Yu, YC Jiang, XZ Li, PF Zhang, ZS Wen, M Zheng
    Cancer Med, 2019-02-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  26. An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.
    Authors: Martinez-Martin N, Marcandalli J, Huang C, Arthur C, Perotti M, Foglierini M, Ho H, Dosey A, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C
    Cell, 2018-07-26;174(5):1158-1171.e19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor
    Authors: WJ Zacharias, DB Frank, JA Zepp, MP Morley, FA Alkhaleel, J Kong, S Zhou, E Cantu, EE Morrisey
    Nature, 2018-02-28;555(7695):251-255.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. VEGF pathway-targeting drugs induce evasive adaptation by activation of neuropilin-1/cMet in colon cancer cells
    Authors: Chisato Tomida, Naoko Yamagishi, Hikaru Nagano, Takayuki Uchida, Ayako Ohno, Katsuya Hirasaka et al.
    International Journal of Oncology
  29. Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma
    Authors: A Lo, CP Li, EL Buza, R Blomberg, P Govindaraj, D Avery, J Monslow, M Hsiao, E Puré
    JCI Insight, 2017-10-05;2(19):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  30. The Effect of Chronic Hepatitis B Virus Infection on BDCA3+ Dendritic Cell Frequency and Function
    PLoS ONE, 2016-08-16;11(8):e0161235.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  31. In vitro system using human neurons demonstrates that varicella-zoster vaccine virus is impaired for reactivation, but not latency
    Authors: T Sadaoka, DP Depledge, L Rajbhandar, A Venkatesan, J Breuer, JI Cohen
    Proc Natl Acad Sci USA, 2016-04-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay - Control
  32. Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL progression
    Oncogene, 2016-02-15;35(36):4741-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  33. EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer
    Authors: Helen Schneck, Berthold Gierke, Frauke Uppenkamp, Bianca Behrens, Dieter Niederacher, Nikolas H. Stoecklein et al.
    PLOS ONE
  34. An Evolutionary-Conserved Function of Mammalian Notch Family Members as Cell Adhesion Molecules
    Authors: Akihiko Murata, Miya Yoshino, Mari Hikosaka, Kazuki Okuyama, Lan Zhou, Seiji Sakano et al.
    PLoS ONE
  35. Highlights of glycosylation and adhesion related genes involved in myogenesis.
    Authors: Grassot V, Da Silva A, Saliba J, Maftah A, Dupuy F, Petit J
    BMC Genomics, 2014-07-22;15(0):621.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  36. Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63.
    Authors: Poeter M, Brandherm I, Rossaint J, Rosso G, Shahin V, Skryabin B, Zarbock A, Gerke V, Rescher U
    Nat Commun, 2014-04-28;5(0):3738.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. Monoclonal antibodies to human laminin alpha4 chain globular domain inhibit tumor cell adhesion and migration on laminins 411 and 421, and binding of alpha6beta1 integrin and MCAM to alpha4-laminins.
    Authors: Ishikawa T, Wondimu Z, Oikawa Y, Ingerpuu S, Virtanen I, Patarroyo M
    Matrix Biol, 2014-03-27;36(0):5-14.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization
  38. Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
    Authors: Kevin Wilhelmsen, Katherine Farrar, Judith Hellman
    Journal of Visualized Experiments
  39. Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR.
    Authors: Stanietsky N, Rovis T, Glasner A, Seidel E, Tsukerman P, Yamin R, Enk J, Jonjic S, Mandelboim O
    Eur J Immunol, 2013-07-04;43(8):2138-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  40. Spatiotemporal Expression of Repulsive Guidance Molecules (RGMs) and Their Receptor Neogenin in the Mouse Brain
    Authors: Dianne M. A. van den Heuvel, Anita J. C. G. M. Hellemons, R. Jeroen Pasterkamp
    PLoS ONE
  41. Cadherin 6 has a functional role in platelet aggregation and thrombus formation.
    Authors: Dunne E, Spring CM, Reheman A, Jin W, Berndt MC, Newman DK, Newman PJ, Ni H, Kenny D
    Arterioscler. Thromb. Vasc. Biol., 2012-04-26;32(7):1724-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  42. The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury.
    Authors: Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X
    PLoS ONE, 2012-04-12;7(4):e34608.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control

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Reviews for Normal Sheep IgG Control

Average Rating: 4.7 (Based on 6 Reviews)

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Normal Sheep IgG Control
By Anonymous on 09/22/2019
Application: WB Sample Tested: Recombinant protein Species: Human
Western Blot Normal Sheep IgG Control 5-001-A
Normal Sheep IgG Control
By Anonymous on 09/04/2019
Application: WB Sample Tested: Recombinant protein Species: Human
Normal Sheep IgG Control
By avinash waghray on 03/11/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult lung Species: Human

no background - with and without retrieval

Immunocytochemistry/Immunofluorescence Normal Sheep IgG Control 5-001-A
Normal Sheep IgG Control
By Anonymous on 05/04/2018
Application: Func Sample Tested: Human recombinant antibody,Human recombinant test antibody Species: Human and Mouse
Normal Sheep IgG Control
By Anonymous on 02/20/2018
Application: Flow Sample Tested: Cancer Cells Species: Human
Flow Cytometry Normal Sheep IgG Control 5-001-A
Normal Sheep IgG Control
By Anonymous on 09/27/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

Great antibody control for ICC

Immunocytochemistry/Immunofluorescence Normal Sheep IgG Control 5-001-A