A23187, free acid

Tocris Bioscience | Catalog # 1234

Calcium ionophore
Tocris Bioscience

Key Product Details

Description

Calcium ionophore

Alternative Names

Calcimycin
View all Calcium Signaling Agents »

Product Description

A23187, free acid is a calcium ionophore that induces Ca2+-dependent cell death by increasing intracellular calcium concentration. Promotes intracelllular ROS generation and platelet particle formation (fragmentation) in vitro and in vivo. Can be used to induce autophagy in mammalian cells.

Product Specifications for A23187, free acid

Molecular Weight

523.63

Formula

C29H37N3O6

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

5-(Methylamino)-2-[[2R,3R,6S,8S,9R,11R)-3,9,11-trimethyl-8-[(1S)-1-methyl-2-oxo-2-(1H-pyrrol-2-yl)-ethyl]-1,7-dioxaspiro[5.5]undec-2-yl]methyl]-4-benzoxazolecarboxylic acid

CAS Number

52665-69-7

PubChem ID

11957499

InChI Key

HIYAVKIYRIFSCZ-CYEMHPAKSA-N

SMILES

[H][C@@]1([C@H](C)C(C5=CC=CN5)=O)[C@H](C)C[C@@H](C)[C@@]2(O[C@H](CC3=NC(C(C(O)=O)=C(NC)C=C4)=C4O3)[C@H](C)CC2)O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 2.62 5

Preparing Stock Solutions for A23187, free acid

The following data is based on the product molecular weight 523.63.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.05 mM 38.19 mL 190.97 mL 381.95 mL
0.25 mM 7.64 mL 38.19 mL 76.39 mL
0.5 mM 3.82 mL 19.10 mL 38.19 mL
2.5 mM 0.76 mL 3.82 mL 7.64 mL

Calculators

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Product Documents for A23187, free acid

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for A23187, free acid

For research use only

Citations for A23187, free acid

Customer Reviews for A23187, free acid (1)

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  • The A23187-induced cytotoxicity cytometrically examined in rat thymocytes
    Name: Lutpine Rachel
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 01/02/2020
    The cytotoxicity of A23187 was attenuated by 1mM EDTA while it was augmented by 50 microM EDTA and 10 microM TPEN.
    A23187 is used to induce Ca(2+)-dependent cell death by increasing intracellular Ca(2+) concentration ([Ca(2+)](i)) under in vitro condition. Since this ionophore also increases membrane permeability of metal divalent cations such as Zn(2+) and Fe(2+) rather than Ca(2+), trace metal cations in cell suspension may affect Ca(2+)-dependent cell death induced by A23187. Therefore, the effects of chelators for divalent metal cations, EDTA and TPEN, on the A23187-induced cytotoxicity were cytometrically examined in rat thymocytes.
    A23187, free acid 1234

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