BDY TMR-X, SE
Chemical Name: (T-4)-[N-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-5-[[5-(4-methoxyphenyl)-2H-pyrrol-2-ylidene-κN]methyl]-2,4-dimethyl-1H-pyrrole-3-propanamidato-κN1]difluoroboron
Purity: ≥95%
Biological Activity
BDY TMR-X, SE is a fluorescent yellow BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Exhibits similar excitation and emission spectra to dye TRITC. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a seven-atom aminohexanoyl ("X") spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between the fluorophore and conjugated biomolecule. The hydrophobic nature of BDY TMR-X makes it ideal for labeling lipids and cell membranes. BDY TMR-X also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~544 nM; emission maximum ~570 nM.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data for BDY TMR-X, SE
| Emission Color | Yellow |
|---|---|
| λabs | 544 nm |
| λem | 570 nm |
| Extinction Coefficient (ε) | 60000 M-1cm-1 |
| Quantum Yield (φ) | 0.96 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.16 |
| Correction Factor 280 | 0.16 |
| Application | Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy |
| Alternative For | 5-TAMRA; Alexa Fluor 555; DyLight 549; TRITC |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Fluorescent exendin-4 derivatives for pancreatic β-cell analysis.
Clardy et al.
Bioconjug.Chem., 2014;25:171
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