DFHBI
Chemical Name: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-4H-imidazol-4-one
Purity: ≥98%
Biological Activity
DFHBI is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 447 nm and peak fluorescence emission of 501 nm when bound to Spinach2.Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
-
In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies.
Filonov et al.
Chem.Biol., 2015;22:649 -
Plug-and-play fluorophores extend the spectral properties of Spinach.
Song et al.
J.Am.Chem.Soc., 2014;136:198 -
Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria.
Strack et al.
Nat.Protoc., 2014;9:146 -
RNA mimics of green fluorescent protein.
Paige et al.
Science, 2011;333:642 -
Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes.
Svensen and Jaffrey
Cell Chem.Biol., 2016;23:415
Product Datasheets
Reconstitution Calculator
Molarity Calculator
FAQs
No product specific FAQs exist for this product, however you may
View all Small Molecule FAQsReviews for DFHBI
There are currently no reviews for this product. Be the first to review DFHBI and earn rewards!
Have you used DFHBI?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
