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DuoSet ELISA Ancillary Reagent Kit 2

R&D Systems | Catalog # DY008B

R&D Systems
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Key Product Details

Applications

ELISA

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Product Summary for DuoSet ELISA Ancillary Reagent Kit 2

Assay Type: ELISA Development Kit
Sufficient Materials: For five 96-well or 384-well plate assays

PRODUCT DETAIL

DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well or 384-well assays. DuoSet Ancillary Kit 2 contains Reagent Diluent 2. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.


PRODUCT FEATURES
  • Complete kit of supplemental supplies provides an economical way to assay five 96-well or 384-well ELISA plates.
  • For use with DuoSets requiring the following Reagent Diluent formulation: 1% BSA in PBS, pH 7.2-7.4, 0.2 micron filtered.
  • Pre-made buffers reduce preparation time and ensure optimal results.

KIT CONTENTS
  • Clear Polystyrene Microplates (five 12x8-well strip microplates, or five 384-well microplates)
  • ELISA Plate Sealers (24 plate sealers)
  • TMB ELISA Substrate (1X, five 12.5 mL vials)
  • Stop Solution (1X, three 11 mL vials)
  • ELISA Plate-coating Buffer (1X, one 60 mL vial)
  • Reagent Diluent Concentrate 2 (10X, two 21 mL vials)
  • Wash Buffer (25X, six 21 mL vials)

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Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Documents for DuoSet ELISA Ancillary Reagent Kit 2

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for DuoSet ELISA Ancillary Reagent Kit 2

For research use only

Citations for DuoSet ELISA Ancillary Reagent Kit 2

Customer Reviews for DuoSet ELISA Ancillary Reagent Kit 2 (17)

4.9 out of 5
17 Customer Ratings
5 Stars
88%
4 Stars
12%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 17 reviews Showing All
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  • Nice product !
    Name: Anonymous
    Sample Tested: Mouse brain tissue
    Verified Customer | Posted 11/21/2025
    TNFa and IL1b standard curves
    I performed an ELISA on brain mouse tissue targeting TNFa and IL1b. I followed the supplier's protocol. We obtain nice reference curves and very nice results.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/29/2025
    all reagents work well for any type of ELISA runs
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Gianna Pavarino
    Verified Customer | Posted 01/22/2025
    Excellent, used for serum samples diluted 1:2 in reagent diluent
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 01/22/2025
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 01/22/2025
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/03/2024
    This Ancillary Reagent Kit provides the necessary reagents for the ELISAs using DuoSet kits such as plates for coating, TMB, and stop solution,...It works excellently.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 05/30/2024
    Excellent collection of reagents. Makes life easier to do the actual assay.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Iona Thomas-Wright
    Verified Customer | Posted 10/30/2023
    This kit came with all the necessary reagents with considerably more than was needed for the five plates so there were no concerns about running out. In combination with the duo-set anti-body pair this made a very nice assay.
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/19/2023
    Color development was quick. Happy with the result and its also comparable to my past data. So trustworthy
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/14/2023
    We used this test kit to assist in the analysis of various functional molecules of TME immune cells, supporting IFN-beta / gamma. The test results were very promising, and the kit provided an ample dosage.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 09/20/2023
    Stable to use in TNF elisa
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 08/20/2023
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 07/05/2023
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Sergi Marco
    Verified Customer | Posted 05/22/2023
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 05/19/2023
    works good with MPO kit
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Andres Ocampo Carrillo
    Verified Customer | Posted 02/24/2023
    The Ancillary was used to conduct more ELISA experiments quantifying TNF-a in mice samples coming from an in vivo model of intestinal inflammation. The kit contains enough reagents as indicated. In the picture, the quantification of TNF-a is presented, comparing the original reagents from the original ELISA, and the quantification using the reagents from the Ancillary Reagent Kit
    DuoSet ELISA Ancillary Reagent Kit 2 DY008B
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/27/2022
    Works great alongside duoset kits

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FAQs for DuoSet ELISA Ancillary Reagent Kit 2

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  • Q: Is the 384-well plate supplied in removable strips?

    A: The 384-well plate is a solid, non-dismountable frame without removable strips.

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