DuoSet ELISA Ancillary Reagent Kit 2
R&D Systems | Catalog # DY008B
Key Product Details
Applications
Assay Type
Format
Product Summary for DuoSet ELISA Ancillary Reagent Kit 2
Sufficient Materials: For five 96-well or 384-well plate assays
PRODUCT DETAIL
DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well or 384-well assays. DuoSet Ancillary Kit 2 contains Reagent Diluent 2. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.
- Complete kit of supplemental supplies provides an economical way to assay five 96-well or 384-well ELISA plates.
- For use with DuoSets requiring the following Reagent Diluent formulation: 1% BSA in PBS, pH 7.2-7.4, 0.2 micron filtered.
- Pre-made buffers reduce preparation time and ensure optimal results.
- Clear Polystyrene Microplates (five 12x8-well strip microplates, or five 384-well microplates)
- ELISA Plate Sealers (24 plate sealers)
- TMB ELISA Substrate (1X, five 12.5 mL vials)
- Stop Solution (1X, three 11 mL vials)
- ELISA Plate-coating Buffer (1X, one 60 mL vial)
- Reagent Diluent Concentrate 2 (10X, two 21 mL vials)
- Wash Buffer (25X, six 21 mL vials)
Formulation, Preparation, and Storage
Shipping
Storage
Product Documents for DuoSet ELISA Ancillary Reagent Kit 2
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for DuoSet ELISA Ancillary Reagent Kit 2
For research use only
Citations for DuoSet ELISA Ancillary Reagent Kit 2
Customer Reviews for DuoSet ELISA Ancillary Reagent Kit 2 (17)
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Customer Images
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Sample Tested: Mouse brain tissueVerified Customer | Posted 11/21/2025TNFa and IL1b standard curvesI performed an ELISA on brain mouse tissue targeting TNFa and IL1b. I followed the supplier's protocol. We obtain nice reference curves and very nice results.
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Verified Customer | Posted 10/29/2025all reagents work well for any type of ELISA runs
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Verified Customer | Posted 01/22/2025Excellent, used for serum samples diluted 1:2 in reagent diluent
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Verified Customer | Posted 01/22/2025
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Verified Customer | Posted 01/22/2025
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Verified Customer | Posted 10/03/2024This Ancillary Reagent Kit provides the necessary reagents for the ELISAs using DuoSet kits such as plates for coating, TMB, and stop solution,...It works excellently.
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Verified Customer | Posted 05/30/2024Excellent collection of reagents. Makes life easier to do the actual assay.
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Verified Customer | Posted 10/30/2023This kit came with all the necessary reagents with considerably more than was needed for the five plates so there were no concerns about running out. In combination with the duo-set anti-body pair this made a very nice assay.
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Verified Customer | Posted 10/19/2023Color development was quick. Happy with the result and its also comparable to my past data. So trustworthy
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Verified Customer | Posted 10/14/2023We used this test kit to assist in the analysis of various functional molecules of TME immune cells, supporting IFN-beta / gamma. The test results were very promising, and the kit provided an ample dosage.
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Verified Customer | Posted 09/20/2023Stable to use in TNF elisa
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Verified Customer | Posted 08/20/2023
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Verified Customer | Posted 07/05/2023
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Verified Customer | Posted 05/22/2023
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Verified Customer | Posted 05/19/2023works good with MPO kit
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Verified Customer | Posted 02/24/2023The Ancillary was used to conduct more ELISA experiments quantifying TNF-a in mice samples coming from an in vivo model of intestinal inflammation. The kit contains enough reagents as indicated. In the picture, the quantification of TNF-a is presented, comparing the original reagents from the original ELISA, and the quantification using the reagents from the Ancillary Reagent Kit
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Verified Customer | Posted 10/27/2022Works great alongside duoset kits
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for DuoSet ELISA Ancillary Reagent Kit 2
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Q: Is the 384-well plate supplied in removable strips?
A: The 384-well plate is a solid, non-dismountable frame without removable strips.