FNIR-Tag, NHS ester
Chemical Name: 2-((E)-2-((E)-2-(3-((4-((2,5-dioxopyrrolidin-1-yl)oxy)-4-oxobutyl)dimethylammonio)propoxy)-3-(2-((E)-1-(2-(2-(2-methoxyethoxy)ethoxy)ethyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)ethylidene)cyclohex-1-en-1-yl)vinyl)-1-(2-(2-(2-methoxyethoxy)ethoxy)ethyl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
Purity: ≥70%
Biological Activity
Key information: FNIR-Tag, NHS ester is a near-infrared fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines with a high degree of labeling (DOL).Application: Designed for in vivo imaging. Also suitable for flow cytometry.
Properties and Photophysical Data: FNIR-Tag, NHS ester provides a higher degree of labeling (DOL) with less aggregation than a leading competitor product when used for antibody conjugation. FNIR-Tag, NHS ester- antibody conjugates exhibit reduced liver uptake in vivo compared with leading competitor conjugates. In addition, FNIR-Tag, NHS ester conjugates are significantly brighter than leading competitor conjugates at similar labeling densities in vivo and in vitro. Antibody conjugation efficiency is greatest at pH 7.4. Excitation and emission maxima (λ) are 766 nM and 788 nM, respectively; quantum yield = 0.099; extinction coefficient = 200,000 M-1cm-1; A280 correction factor = 0.05.
For further information about how FNIR-Tag, NHS ester may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Scientific Data
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Application of FNIR-Tag, NHS ester in vitro. C166 mouse endothelial cells were incubated with 20 nM virus-like particles (VLPs) at 37 °C for 1 h. Samples were extensively washed to remove VLPs not bound to the cellular surface and subsequently fixed to preserve any interactions prior to analysis by flow cytometry. FNIR-Tag conjugates (red) were significantly brighter than IR-800CW conjugates (black) at similar labeling at low (A) and high (B) fluorophore loadings. For more information on this work read Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512
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Application of FNIR-Tag, NHS ester in vivo. (A) Representative images demonstrating detection of virus-like particle (VLP) conjugates in the liver at 2 h (left panel) and 24 h (right panel) post injection. Scale bar represents epifluorescent in total radiant efficiency. (B) Quantitative analysis of the fluorescence intensity detected at 2 and 24 h post injection. Data are mean value ± standard error. Statistical analysis was performed between groups at the same time point using Student’s t-test. *p ≤ 0.05; **p ≤ 0.01. For more information on this work read Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512
Optical Data for FNIR-Tag, NHS ester
| Emission Color | Near-IR |
|---|---|
| Brightness | |
| λabs | 765 nm |
| λem | 788 nm |
| Extinction Coefficient (ε) | 200,000 M-1cm-1 |
| Quantum Yield (φ) | 0.099 |
| Closest Laser line | 750 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Correction Factor 280 | 0.05 |
| Application | Flow Cytometry, In Vivo Imaging |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
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