FURA-2AM
Chemical Name: 2-[6-[bis[2-[(Acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl ester
Purity: ≥95%
Biological Activity
FURA-2AM is a fluorescent ratiometric Ca2+ indicator (Kd Ca2+ = 145 nM). FURA-2AM is selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. FURA-2AM binds to free intracellular calcium and is used to determine [Ca2+]i concentration.This product is typically prepared in DMSO. F 127 (Cat. No. 6253) for the solubilization of FURA-2AM is also available.
Optical Data for FURA-2AM
| λabs | 371 nm |
|---|---|
| λem | 474 nm |
| Extinction Coefficient (ε) | 30000 M-1cm-1 |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Fluorescent Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Characterisation of AmphiAmR11, an amphioxus (Branchiostoma floridae) D2-dopamine-like G protein-coupled receptor.
Bayliss A, Evans P
PLoS ONE, 2013;8(11):e80833. -
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
Grynkiewicz et al.
J.Biol.Chem., 1985;260:3440 -
Sildenafil inhibits human pulmonary artery smooth muscle cell proliferation by decreasing capacitative Ca2+ entry.
Wang et al.
J.Pharmacol.Sci., 2008;108:71
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Citations for FURA-2AM
The citations listed below are publications that use Tocris products. Selected citations for FURA-2AM include:
3 Citations: Showing 1 - 3
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Niclosamide repurposed for the treatment of inflammatory airway disease.
Authors: Cabrita Et al.
JCI Insight 2019;4
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KA attenuates the Na+-dependent Ca2+ overload in rabbit ventricular myocytes in vitro by inhibiting late Na+ and L-type Ca2+ currents.
Authors: Luo Et al.
Mol Neurodegener 2015;36:1327
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Characterisation of AmphiAmR11, an amphioxus (Branchiostoma floridae) D2-DA-like G protein-coupled receptor.
Authors: Bayliss and Evans
PLoS One 2013;8:e80833
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