R&D Systems offers high quality Recombinant Heparinase I, II, and III at competitive pricing.
Heparin and heparan sulfate are sulfated glycosaminoglycans that share basic carbohydrate backbone structure with alternating uronic acid and N-acetylglucosamine residues. Heparin is found in mast cells and has strong anticoagulation properties. Heparan sulfate is found on cell membrane and extracellular matrix and is involved in various activities from cell growth, adhesion, and migration to lipid metabolism.
Heparin has a higher degree of sulfation than heparan sulfate, and both can be digested by heparinases, a group of bacterial lyases that are widely used as tools for processing and analysis.
Heparinase I
Heparinase I from F. heparinum recognizes highly sulfated regions and exhibits greater activity on heparin compared to heparan.
| Heparinase I | Specific Activity |
| R&D Systems | 21074.9 pm/min/µg |
| Assay Conditions: Heparinase I amount: 0.188 µg, Substrate (Heparin Sulfate): 200 µg Assay Buffer: 20 mM Tris, 100 mM NaCl, 1.5 mM CaCl2, pH 7.0 Reaction Conditions: Room Temperature, 5 min Kinetic Assay |
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Heparinase I digestion of Heparin Sulfate
(200 µg) is assessed in a 5-minute kinetic assay at room temperature by monitoring absorbance at 232 nm.
Heparinase II
Heparinase II from P. heparinus exhibits digests both heparin and heparan sulfate.
| Heparinase II | Specific Activity |
| R&D Systems | 2595.6 pm/min/µg |
| Assay Conditions: Heparinase II amount: 0.475 µg, Substrate (Heparin Sulfate): 200 µg Assay Buffer: 20 mM Tris, 100 mM NaCl, 1.5 mM CaCl2, pH 7.0 Reaction Conditons: Room Temperature, 5 min Kinetic Assay |
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Heparinase II digestion of Heparin Sulfate
(200 µg) is assessed in a 5-minute kinetic assay by monitoring absorbance at 232 nm.
Heparinase III
Heparinase III from P. heparinus exhibits specificity for less-sulfated regions and is more active on heparan sulfate than heparin sulfate.
| Heparinase III | Specific Activity |
| R&D Systems | 7454.8 pm/min/µg |
| Assay Conditions: Heparinase III amount: 0.759 µg, Substrate (Heparan Sulfate): 200 µg Assay Buffer: 20 mM Tris, 100 mM NaCl, 1.5 mM CaCl2, pH 7.0 Reaction Conditons: Room Temperature, 5 min Kinetic Assay |
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Heparinase III digestion of Heparan Sulfate
(200 µg) is assessed in a 5-minute kinetic assay at room temperature by monitoring absorbance at 232 nm.