Human Activin B MAb (Clone 146807) Summary
Gly293-Ala407
Accession # Q53T31
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Hemoglobin Expression Induced by Activin B and Neutralization by Human Activin B Antibody. Recombinant Human Activin B beta B subunit (Catalog # 659-AB) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Human Activin B beta B subunit (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Activin B beta B subunit Monoclonal Antibody (Catalog # MAB659). The ND50 is typically 0.6-3.0 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Activin B
Activins and inhibins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins that were originally purified from gonadal fluids as proteins that stimulated or inhibited, respectively, pituitary follicle stimulating hormone (FSH) release. These proteins have since been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and reproductive physiology. Activins/inhibins are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release the carboxy-terminal bioactive ligands. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned. The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A ( beta A‑ beta A), activin B ( beta B‑ beta B), activin AB ( beta A‑ beta B), inhibin A ( alpha ‑ beta A) and inhibin B ( alpha ‑ beta B). At present, little is known about the contribution of the other beta subunits to activin or inhibin formation and biology. At the amino acid sequence level, the mature human beta B subunit is greater than 98% identical to mouse beta B, while the human and mouse alpha subunits share approximately 80% identity. Similarly to other TGF-beta family members, activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated as type I and type II. Two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B) have been identified. Activin binds directly to Act RII, the complex then associates with Act RI and initiates signaling. Besides activins, Act RII has been shown to bind certain other TGF-beta superfamily members. Inhibin A has been shown to bind with low-affinity to Act RII. The existence of a distinct inhibin-specific receptor and/or signal transduction pathway has been hypothesized.
- Woodruff, T.K. (1998) Biochemical Pharmacology 55:953.
- Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114.
Product Datasheets
Citations for Human Activin B MAb (Clone 146807)
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Activin B and Activin C Have Opposing Effects on Prostate Cancer Progression and Cell Growth
Authors: KL Reader, S John-McHaf, S Zellhuber-, T Jowett, DG Mottershea, HE Cunliffe, EJ Gold
Cancers, 2022-12-27;15(1):.
Species: Human
Sample Types: Tissue Array
Applications: IHC -
An autocrine ActivinB mechanism drives TGF?/Activin signaling in Group 3 medulloblastoma
Authors: M Morabito, M Larcher, FM Cavalli, C Foray, A Forget, L Mirabal-Or, M Andrianter, S Druillenne, A Garancher, J Masliah-Pl, S Leboucher, A Debalkew, A Raso, O Delattre, S Puget, F Doz, MD Taylor, O Ayrault, F Bourdeaut, A Eychène, C Pouponnot
EMBO Mol Med, 2019-07-22;0(0):e9830.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
An activin receptor IIA ligand trap corrects ineffective erythropoiesis in beta-thalassemia.
Authors: Dussiot M, Maciel T, Fricot A, Chartier C, Negre O, Veiga J, Grapton D, Paubelle E, Payen E, Beuzard Y, Leboulch P, Ribeil J, Arlet J, Cote F, Courtois G, Ginzburg Y, Daniel T, Chopra R, Sung V, Hermine O, Moura I
Nat Med, 2014-03-23;20(4):398-407.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC-P, Neutralization -
The neuroprotective effect of Activin A and B: implication for neurodegenerative diseases.
Authors: Kupershmidt L, Amit T, Bar-Am O, Youdim MB, Blumenfeld Z
J. Neurochem., 2007-08-06;103(3):962-71.
Species: Human, Rat
Sample Types: Cell Lysates
Applications: Western Blot -
The role of activins in hepcidin regulation during malaria
Authors: N Spottiswoo, AE Armitage, AR Williams, AJ Fyfe, S Biswas, SH Hodgson, D Llewellyn, P Choudhary, SJ Draper, P Duffy, H Drakesmith
Infect. Immun., 2017-11-17;0(0):.
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