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Human Activin RIIA Antibody

Catalog #: MAB340
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB340 has been discontinued.
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Detection of Human Activin RIIA by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Activin RIIA Antibody Summary

Species Reactivity
Human
Specificity
Detects human Activin RIIA in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) Activin RIA, rhActivin RIB, or rhActivin RIIB.
Source
Monoclonal Mouse IgG2A Clone # 126706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Activin RIIA
Ala20-Pro134
Accession # P27037
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Activin RIIA antibody by Western Blot. View Larger

Detection of Human Activin RIIA by Western Blot. Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Activin RIIA Monoclonal Antibody (Catalog # MAB340) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Activin RIIA at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIIA

Activin isoforms and other members of the TGF-beta  superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A) and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists.

References
  1. Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066.
  2. Woodruff, T.K. (1998) Biochem. Pharmacology, 55:953.
Long Name
Activin Receptor IIA
Entrez Gene IDs
92 (Human); 11480 (Mouse)
Alternate Names
activin A receptor, type II; activin A receptor, type IIA; Activin receptor type IIA; activin receptor type-2A; Activin RIIA; ActivinRIIA; ACTRII; ACTRIIA; ACVR2A; ACVR2ACTR-IIA; AVR2A; EC 2.7.11; EC 2.7.11.30

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Citation for Human Activin RIIA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
    Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
    J. Mol. Biol., 2008-07-02;381(4):1025-39.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development

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