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Human alpha-Synuclein Antibody

Catalog #: MAB13383 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB13383-100
MAB13383-SP
Detection of Human alpha -Synuclein by Western Blot.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha-Synuclein Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha -Synuclein in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 970114
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human alpha -Synuclein
Met1-Ala140
Accession # P37840
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
5 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human a-Synuclein antibody by Western Blot. View Larger

Detection of Human alpha -Synuclein by Western Blot. Western blot shows lysates of human brain (motor cortex and cerebellum) tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human a-Synuclein Monoclonal Antibody (Catalog # MAB13383) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry a-Synuclein antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

alpha -Synuclein in Human Brain. a-Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human a-Synuclein Monoclonal Antibody (Catalog # MAB13383) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Synuclein

alpha -Synuclein is a cytoplasmic protein that is predominantly expressed in the central nervous system. It is a precursor of a minor peptide found in amyloid deposition that is a hallmark of Alzheimer's disease. Defects in alpha -Synuclein are associated with familial Parkinson's disease.

Entrez Gene IDs
6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)
Alternate Names
alpha-Synuclein; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha; truncated alpha synuclein

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Human alpha-Synuclein Antibody
By Anonymous on 06/17/2022
Application: IHC Sample Tested: Brain tissue Species: Human
Immunohistochemistry Human alpha-Synuclein Antibody MAB13383