Human Autoimmune Regulator/AIRE Antibody

Name: Human Autoimmune Regulator/AIRE Antibody
Brand: R&D Systems
SKU: MAB5936

Catalog #: MAB5936 Datasheet / COA / SDS

Discontinued Product

MAB5936 has been discontinued.

View all Autoimmune Regulator/AIRE products.

Detection of Autoimmune Regulator/AIRE in Human PBMC by Flow Cytometry.

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All Autoimmune Regulator/AIRE Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Autoimmune Regulator/AIRE Antibody Summary

Species Reactivity

Human

Specificity

Detects human Autoimmune Regulator/AIRE in direct ELISAs. In Western blots, no cross-reactivity with recombinant mouse AIRE is observed.

Source

Monoclonal Mouse IgG₁ Clone # 614530

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human Autoimmune Regulator/AIRE
Ser476-Ser545
Accession # O43918

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Autoimmune Regulator/AIRE antibody in Human PBMC antibody by Flow Cytometry.

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Detection of Autoimmune Regulator/AIRE in Human PBMC by Flow Cytometry. Human peripheral blood monocytes were stained with Human Autoimmune Regulator/AIRE Monoclonal Antibody (Catalog # MAB5936, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Autoimmune Regulator/AIRE

AutoImmune REgulator (AIRE) is an approximately 60 kDa nuclear and cytosolic protein that is required for the development of T cell tolerance. It regulates the expression of self-antigens by thymic epithelial cells, and mutations in AIRE are causative of the autoimmune disorder, APECED. AIRE regulates gene transcription through interactions with DNA, histone H3, and the nuclear matrix. It contains one HSD domain (aa 1-105), a nuclear localization sequence (aa 113-133), one SAND domain (aa 181-280), and two PHD zinc finger domains (aa 299-340 and aa 434-475). Alternate splicing of human AIRE generates isoforms that lack the HSR and SAND domains and/or the second PHD domain. Within aa 476-545, human AIRE shares 65% and 63% aa sequence identity with mouse and rat AIRE, respectively.

Entrez Gene IDs

326 (Human); 11634 (Mouse); 294328 (Rat)

Alternate Names

AIRE; APECED protein; APECED; APECEDAPSI; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune regulator (APECED protein)10APS1AIRE1; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy); Autoimmune Regulator; PGA1