Human Carbonic Anhydrase III/CA3 Biotinylated Antibody

Catalog #: BAF2185 Datasheet / COA / SDS

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BAF2185

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All Carbonic Anhydrase III/CA3 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase III/CA3 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbonic Anhydrase III/CA3 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) CA1, 2, 4, 8, 9, 10 and 14 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase III/CA3
Ala2-Lys260
Accession # P07451

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Carbonic Anhydrase III (Catalog # 2185-CA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase III/CA3

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1).

Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50‑60% of adult levels at birth (2).

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.

Entrez Gene IDs

761 (Human)

Alternate Names

CA3; CAIII; CA-III; Car3; Carbonate dehydratase III; carbonic anhydrase 3; Carbonic Anhydrase III; carbonic anhydrase III, muscle specific; EC 4.2.1.1