Human CD21 Antibody Summary
Ile21-Arg971
Accession # P20023
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human CD21 by Western Blot. Western blot shows lysates of human spleen tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human CD21 Monoclonal Antibody (Catalog # MAB4909) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CD21 at approximately 145 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1. This antibody is recommended to be used under non-reducing conditions only.
View Larger
Detection of CD21 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD21 Monoclonal Antibody (Catalog # MAB4909) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD21
CD21 (also called EBV receptor or CR2) is a 145 kDa member of the RCA (receptors of complement activation) family of proteins. It is expressed on T cells, B cells, and follicular dendritic cells. On the B cell surface, it combines with the BCR and CD19 to form a B cell activating complex. Mature human CD21 is 1013 amino acids (aa) in length. It is a type I transmembrane (TM) protein that contains a 951 aa extracellular domain (ECD) (aa 21-971) and a short 34 aa cytoplasmic tail. The ECD exhibits fifteen 60 aa SUSHI repeats. Soluble CD21 can be generated by cleavage near the TM domain. One potential splice variant shows a deletion of aa 847‑908, while another shows an insertion of 59 aa after Lys659. The ECD of human CD21 shares 71% aa identity with mouse CD21 ECD.
Product Datasheets
Citations for Human CD21 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis.
Authors: Komori M, Blake A, Greenwood M et al.
Ann Neurol
-
HLA-DQ ?1 alleles associated with Epstein-Barr virus (EBV) infectivity and EBV gp42 binding to cells
Authors: Q Li, W Bu, E Gabriel, F Aguilar, Y Hoshino, H Miyadera, C Hess, RL Hornung, A Roy, JI Cohen
JCI Insight, 2017-02-23;2(4):e85687.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Insufficient disease inhibition by intrathecal rituximab in progressive multiple sclerosis
Authors: Mika Komori, Yen Chih Lin, Irene Cortese, Andrew Blake, Joan Ohayon, Jamie Cherup et al.
Annals of Clinical and Translational Neurology
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human CD21 Antibody
There are currently no reviews for this product. Be the first to review Human CD21 Antibody and earn rewards!
Have you used Human CD21 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
