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Human CD25/IL-2R alpha Antibody

Catalog #: AF-223-NA
6 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-223-NA
AF-223-SP
Detection of Human CD25/IL-2R alpha by Western Blot.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD25/IL-2R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD25/IL‑2 R alpha in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 µg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
Human whole blood lymphocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
CD25/IL-2R alpha is specifically detected in HDLM‑2 human Hodgkin's lymphoma cell line parental cell line but is not detectable in CD25/IL-2R alpha knockout HDLM‑2 human Hodgkin's lymphoma cell line cell line.
 
Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD25/IL-2R alpha antibody by Western Blot. View Larger

Detection of Human CD25/IL-2R alpha by Western Blot. Western blot shows lysates of HDLM-2 human Hodgkin's lymphoma cell line and PC-3 human prostate cancer cell line (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD25/IL-2R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated). GAPDH (AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated View Larger

Western Blot Shows Human CD25/IL-2R alpha Specificity by Using Knockout Cell Line. Western blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line and human CD25/IL-2 R alpha knockout HDLM‑2 human Hodgkin’s lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD25/IL-2R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated) in the parental HDLM‑2 human Hodgkin’s lymphoma cell line cell line, but is not detectable in knockout HDLM‑2 human Hodgkin’s lymphoma cell line cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Neutralization Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody. View Larger

Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody. Recombinant Human IL-2 (202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA). The ND50 is typically 0.2-1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CD25/IL-2R alpha

Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

Long Name
Interleukin 2 Receptor alpha
Entrez Gene IDs
3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names
CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

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Citations for Human CD25/IL-2R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Endogenous factors enhance HIV infection of tissue naive CD4 T cells by stimulating high molecular mass APOBEC3G complex formation
    Authors: Jason F. Kreisberg, Wes Yonemoto, Warner C. Greene
    The Journal of Experimental Medicine
  2. Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
    Authors: Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
    J. Immunol., 2008-06-01;180(11):7221-9.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  3. Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab.
    Authors: Binder M, Vogtle FN, Michelfelder S, Muller F, Illerhaus G, Sundararajan S, Mertelsmann R, Trepel M
    Cancer Res., 2007-04-15;67(8):3518-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Interleukin-2alpha receptor in membrane lipid rafts.
    Authors: Li QR, Ma J, Wang H, Li JS
    Transplant. Proc., 2005-06-01;37(5):2395-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses.
    Authors: Sandrin V, Muriaux D, Darlix JL, Cosset FL
    J. Virol., 2004-07-01;78(13):7153-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Horses with equine recurrent uveitis have an activated CD4+ T‐cell phenotype that can be modulated by mesenchymal stem cells in vitro
    Authors: Laurel K. Saldinger, Seldy G. Nelson, Rebecca R. Bellone, Mary Lassaline, Maura Mack, Naomi J. Walker et al.
    Veterinary Ophthalmology

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Reviews for Human CD25/IL-2R alpha Antibody

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Human CD25/IL-2R alpha Antibody
By Katie Myers on 11/28/2022
Application: ELISA Sample Tested: Serum Species: Primate

Used as capture antibody in NHP ELISA

ELISA Human CD25/IL-2R alpha Antibody AF-223-NA
Human CD25/IL-2R alpha Antibody
By Anonymous on 07/23/2019
Application: ELISA Sample Tested: EDTA Plasma Species: Human
ELISA Human CD25/IL-2R alpha Antibody AF-223-NA
Human CD25/IL-2 R alpha Antibody
By Chris Lyman on 11/03/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody served as both a capture and detection antibody in a sandwich ELISA developed to measure human serum and plasma samples.

ELISA Human CD25/IL-2 R alpha Antibody AF-223-NA