Human CD28 Antibody

Name: Human CD28 Antibody
Brand: R&D Systems
SKU: MAB11412
Price: 419 USD
Availability: InStock

R&D Systems | Catalog # MAB11412

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Product Documents

GMP Version Available: MAB11412-GMP

GMP

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

T Cell Stimulation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG₁ Clone # 29704-1

View all CD28 Primary Antibodies »

Product Specifications

Immunogen

Human T lymphocytes

Specificity

Detects human CD28 in direct ELISAs.

Clonality

Monoclonal

Host

Human

Isotype

IgG₁

Scientific Data Images for Human CD28 Antibody

Recombinant Human CD28 Antibody Enhances IL-2 Secretion in Jurkat Cells.

Human CD28 recombinant antibody enhances IL-2 secretion in Jurkat cells (immortalized human T cell line) treated with 5 ng/mL phorbol myristate acetate (PMA) and 0.9 µM calcium ionomycin. Antibody-mediated stimulation occurred in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA kit. The ED₅₀ for this effect is 5-50 ng/mL.

Applications for Human CD28 Antibody

Application

Recommended Usage

T Cell Stimulation

3 µg/mL
Sample: Human PBMCs

Formulation, Preparation, and Storage

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD28

The Bio-Techne CD28 GMP antibody is derived from the 15e8 clone. CD28 is a critical protein expressed on the surface of T cells that provides a co-stimulatory signal necessary for T cell activation and proliferation. CD28 is structurally similar to CTLA-4, with both molecules exhibiting structural homology to the immunoglobulin (Ig) gene superfamily. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. Both CD28 and CTLA-4 are composed of a single Ig V-like extracellular domain, a transmembrane domain, and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2.