Human CD39/ENTPD1 Antibody

Catalog #: MAB11582 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11582-100
MAB11582-SP

Detection of CD39/ENTPD1 in PBMCs by Flow Cytometry

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All CD39/ENTPD1 Antibodies

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Human CD39/ENTPD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human CD39 protein in Direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2991A

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human CD39/ENTPD1
Thr38-Val478
Accession # P49961

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

see below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD39/ENTPD1 in PBMCs by Flow Cytometry PBMCs were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Rabbit Anti-Human CD39/ENTPD1 Monoclonal Antibody (Catalog # MAB11582) or (B) isotype control antibody (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. rhNTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble rhNTPDase-1 ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (2), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (3). NTPDase-1 hydrolyzes the beta - and gamma phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (4). NTPDase-1 is involved in the processes of thrombo regulation and vascular inflammation (5). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (6).

References

Maliszewski, C.R. et al. (1994) J. Immunol. 153:3574.
Rowe, M. et al. (1982) Int. J. Cancer 29:373.
Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 1

Entrez Gene IDs

953 (Human); 12495 (Mouse); 64519 (Rat); 102132860 (Cynomolgus Monkey)

Alternate Names

ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1