Human CD5 PE-conjugated Antibody

Name: Human CD5 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB1636P

Catalog #: FAB1636P Datasheet / COA / SDS

Discontinued Product

FAB1636P has been discontinued.

View all CD5 products.

Detection of CD5 in Human Blood Lymphocytes by Flow Cytometry.

1 Image

All CD5 Antibodies

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD5 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD5 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 205919

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD5
Arg25-Asn371
Accession # P06127

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD5 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD5 PE-conjugated Monoclonal Antibody (Catalog # FAB1636P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD5

CD5, also known as Leu-1, is a type I transmembrane protein belonging to the Scavenger Receptor Cysteine-rich (SRCR) superfamily. It is expressed on thymocytes, T cells and the B1a subpopulation of B cells. CD5 binds the C-type lectin, CD72, and modulates signaling through the antigen receptors on T and B cells. CD5 has been shown to provide either positive or negative co-stimulatory signals depending on cell type to regulate immune responses.

References