Human CD6 Antibody

Catalog #: MAB627
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB627
MAB627-SP

All CD6 Antibodies

Product Details

Citations (1)

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Human CD6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD6 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 123119

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD6
His18-Glu398
Accession # CAA43306

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CD6 Fc Chimera (Catalog # 627-CD)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD6

CD6 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 is a type I membrane glycoprotein and contains three extracellular SRCR domains. CD6 is expressed at low levels on immature thymocytes and at high levels on mature thymocytes. The majority of peripheral blood T cells, a subset of B cells, and a subset of neuronal cells express CD6. CD6 is a 668 amino acid protein with a 24 amino acid predicted signal sequence, a 374 amino acid extracellular domain, and a 244 amino acid transmembrane region. The 626 amino acid murine homolog has also been identified. The human and murine proteins share 70% amino acid identity over their full‑lengths.

CD6 appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6 specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti-CD2 mAb, support the concept of CD6 as a co-stimulatory molecule. Anti-CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6⁺ T cells from donor bone marrow prior to allogeneic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6, it has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development. The presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6⁺ T cells and ALCAM⁺ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction. Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti-CD6 mAb in conjunction with PMA, anti-CD2, or anti-CD3 mAb. The CD6 intracellular domain contains regions that can interact with SH2 or SH3 containing proteins.

References

Gangemi, R.M. et al. (1989) J. Immunol. 143:2439.
Aruffo, A. et al. (1991) J. Exp. Med. 174:949.
Swack, J.A. et al. (1991) J. Biol. Chem. 266:7137.
Robinson, W.H. et al. (1995) Eur. J. Immunol. 25:2765.
Whitney, G. et al. (1995) Mol. Immunol. 32:89.
Starling, G.C. et al. (1996) Eur. J. Immunol. 26:738.
Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
Swack, J.A. et al. (1989) Mol. Immunol. 26:1037.
Pawelec, G. and H.J. Buhring (1991) Hum. Immunol. 31:165.
Osorio, L.M. et al. (1995) Cell Immunol. 166:44.
Robinson, W.H. et al. (1995) J. Immunol. 155:4739.
Singer, N.G. et al. (1996) Immunology 88:537.
Aruffo, A. et al. (1997) Immunol. Today 18:498.

Entrez Gene IDs

923 (Human); 12511 (Mouse); 102143944 (Cynomolgus Monkey)

Alternate Names

CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6

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Citation for Human CD6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1