Human Chk1 Antibody

Name: Human Chk1 Antibody
Brand: R&D Systems
SKU: MAB1630

Catalog #: MAB1630 Datasheet / COA / SDS

Discontinued Product

MAB1630 has been discontinued.

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All Chk1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chk1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chk1.

Source

Monoclonal Mouse IgG_2B Clone # 251903

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Chk1
Tyr157-Thr476
Accession # O14757

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Immunoprecipitation

2.5 µg/500 µg cell lysate

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunoprecipitation

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Immunoprecipitation of Human Chk1. HeLa human cervical epithelial carcinoma cell line was untreated (-) or exposed (+) to 50 J/m²UV light for 1 hour. Chk1 was immunoprecipitated from 500 µg of cell lysate following incubation with 2.5 µg Mouse Anti-Human Chk1 Monoclonal Antibody (Catalog # MAB1630) for 1 hour on ice. Chk1-antibody complexes were absorbed using goat anti-mouse agarose (Sigma). Immunoprecipitated Chk1 (left panel) was detected by Western blot using 1 µg/mL Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U ?-phosphatase (?-PPase) for 1 hour. For additional reference, Western blot (right panel) shows lysates of HeLa cell line probed with 1 µg/mL Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054). View our recommended buffer recipes for immunoprecipitation.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chk1

The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic stress, Chk1 is phosphorylated by ATM or ATM-related kinases. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.

Long Name

Checkpoint Kinase 1

Entrez Gene IDs

1111 (Human); 12649 (Mouse); 140583 (Rat)

Alternate Names

Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1 (checkpoint, S.pombe) homolog; CHK1 checkpoint homolog (S. pombe); Chk1; CHK1serine/threonine-protein kinase Chk1; EC 2.7.11; EC 2.7.11.1