Human Coagulation Factor III/Tissue Factor PerCP-conjugated Antibody

Name: Human Coagulation Factor III/Tissue Factor PerCP-conjugated Antibody
Brand: R&D Systems
SKU: FAB23391C

Catalog #: FAB23391C Datasheet / COA / SDS

Discontinued Product

FAB23391C has been discontinued.

View all Coagulation Factor III/Tissue Factor products.

Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry.

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All Coagulation Factor III/Tissue Factor Antibodies

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Human Coagulation Factor III/Tissue Factor PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.

Source

Monoclonal Mouse IgG₁ Clone # 323519

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III
Gly34-Glu251
Accession # P13726

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor PerCP-conjugated Monoclonal Antibody (Catalog # FAB23391C) or (B) Mouse IgG₁PerCP Isotype Control (Catalog # IC002C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a 46 kDa type I transmembrane protein that is part of the extrinsic pathway of coagulation. It functions as a protein cofactor/receptor for Coagulation Factor VII, which is synthesized in the liver and circulates in the plasma (1). Upon binding to TF, the inactive Factor VII is rapidly converted into activated Factor VIIa. The resulting 1:1 complex of Factor VIIa and TF activates the coagulation pathway, and, as a soluble form, plays an important coagulation-independent role in processes such as angiogenesis and M1 macrophage development (2-5). TF is synthesized as a 295 amino acid (aa) precursor that consists of a signal peptide (aa 1-32) plus a mature domain (aa 33-295). The mature region contain an extracellular region (aa 33-251), followed by a transmembrane segment (aa 252-274) and a cytoplasmic tail (aa 275-295) (6-9). TF is found on endothelial cells, monocytes, vascular smooth muscle cells, and bronchial epithelium (5, 10-12). Over aa 33-251, human and mouse TF share 58% aa sequence identity.

References

Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659.
Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
Gedding, J.E. and N. Mackman (2014) Thromb. Haemost. 111:570.
Hobbs, J.E. et al. (2007) Thromb. Res. 120 Suppl 2:S13.
Venkatasubramanian, S, et al. (2015) Eur. J. Immunol. Oct 16:e201545817.
Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
Morrissey, J.H. et al. (1987) Cell 50:129.
Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
Yang, X. et al. (2016) Thromb. Res. 137:211.
Arderiu, G. et al. (2015) Arterioscler. Thromb. Vasc. Biol. 35:348.
Mitchel, J.A. et al. (2015) Am. J. Resp. Cell Mol. Biol. Sept 25 [ePub ahead of print].

Entrez Gene IDs

2152 (Human); 14066 (Mouse)

Alternate Names

CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor