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Human CXCL13/BLC/BCA-1 APC-conjugated Antibody

Catalog #: IC801A
5 Citations Datasheet / COA / SDS

Discontinued Product

IC801A has been discontinued.
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Detection of CXCL13/BLC/BCA‑1 in Human Immature Dendritic Cells by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCL13/BLC/BCA-1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL13/BLC/BCA-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL1, rhCXCL2, rhCXCL3, rhCXCL8/IL-8, rhCXCL10, rhCXCL9, rhCXCL12/SDF-1 alpha, or rhCXCL12/SDF-1 beta.
Source
Monoclonal Mouse IgG1 Clone # 53610
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CXCL13/BLC/BCA-1
Val23-Arg94
Accession # O43927
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CXCL13/BLC/BCA-1 antibody in Human Immature Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of CXCL13/BLC/BCA‑1 in Human Immature Dendritic Cells by Flow Cytometry. Human immature dendritic cells (CD14+ PBMC cultured with 20 ng/mL rhIL-4 and 50 ng/mL rhGM-CSF for 7 days) were stained with Mouse Anti-Human CXCL13/BLC/BCA-1 APC-conjugated Monoclonal Antibody (Catalog # IC801A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References
  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.
Entrez Gene IDs
10563 (Human); 55985 (Mouse)
Alternate Names
ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

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Citations for Human CXCL13/BLC/BCA-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. The immune landscape of human thymic epithelial tumors
    Authors: Z Xin, M Lin, Z Hao, D Chen, Y Chen, X Chen, X Xu, J Li, D Wu, Y Chai, P Wu
    Nature Communications, 2022-09-17;13(1):5463.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CXCL13-producing CD4+ T cells accumulate in early phase of tertiary lymphoid structures in ovarian cancer
    Authors: M Ukita, J Hamanishi, H Yoshitomi, K Yamanoi, S Takamatsu, A Ueda, H Suzuki, Y Hosoe, Y Furutake, M Taki, K Abiko, K Yamaguchi, H Nakai, T Baba, N Matsumura, A Yoshizawa, H Ueno, M Mandai
    JCI Insight, 2022-06-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments
    Authors: H Yoshitomi, S Kobayashi, A Miyagawa-H, A Okahata, K Doi, K Nishitani, K Murata, H Ito, T Tsuruyama, H Haga, S Matsuda, J Toguchida
    Nat Commun, 2018-09-19;9(1):3762.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells.
    Authors: Kobayashi S, Watanabe T, Suzuki R, Furu M, Ito H, Ito J, Matsuda S, Yoshitomi H
    Eur J Immunol, 2015-11-24;46(2):360-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. HIV-1 single-stranded RNA induces CXCL13 secretion in human monocytes via TLR7 activation and plasmacytoid dendritic cell-derived type I IFN.
    Authors: Cohen K, Dugast A, Alter G, McElrath M, Stamatatos L
    J Immunol, 2015-02-09;194(6):2769-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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