Human DMP-1 Antibody

Catalog #: MAB4129
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4129
MAB4129-SP

All DMP-1 Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DMP-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DMP-1 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse DMP-1, recombinant human (rh) CD44, and rhComplement Factor H is observed.

Source

Monoclonal Mouse IgG₁ Clone # 560716

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DMP-1 isoform 2
Leu17-Tyr497
Accession # NP_001073380

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Human DMP-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human DMP‑1 Biotinylated Antibody (Catalog # BAM41291)

Standard: Recombinant Human DMP-1 Protein, CF (Catalog # 4129-DM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DMP-1

Dentin matrix protein 1 (DMP-1) is a member of the SIBLING family that also includes bone sialoprotein, dentin sialophosphoprotein, MEPE, and osteopontin. These highly phosphorylated integrin-binding proteins are rich in acidic amino acids and function in the formation of calcified bone and tooth matrix (1, 2). The phosphate content, spacing of acidic residues, and calcium-dependent dimerization of DMP-1 contribute to its ability to sequester calcium phosphate clusters and promote hydroxyapatite (HA) crystal formation (3-5). Rodent DMP-1 is cleaved by BMP-1 family proteases at a single site which is conserved in human, generating a 37 kDa N-terminal and a 57 kDa C-terminal fragment (6). The N-terminal fragment in rat carries chondroitin sulfate (7). The C-terminal fragment alone can nucleate HA crystals, while crystal growth into a needle-like morphology is inhibited by the N-terminal fragment (3, 4). Crystal maturation is dependent on the presence of type I collagen (4). DMP-1 is required for odontoblast differentiation as well as dentin formation (8). Nonphosphorylated DMP-1 is targeted to the nucleus, where it activates the transcription of odontoblast and osteoblast specific genes (9, 10). Early in osteoblast maturation, nuclear DMP-1 is extensively phosphorylated by casein kinase II, triggering its secretion (9). DMP-1 mutations in humans are associated with hypophosphatemia and FGF23 overexpression (11, 12). DMP-1 induces the activation of proMMP-9 and displaces mature MMP-9 from TIMP1 (13). DMP-1 tethering of MMP-9 to the cell surface via CD44 and integrins alpha v beta 3 and alpha v beta 5 promotes tumor cell invasiveness in vitro (14). Full length DMP-1 circulates in human serum in a tight complex with complement factor H (13, 14). When first bound to CD44 or integrin alpha v beta 3, DMP-1 can anchor factor H to the cell surface and protect the cell from complement-mediated lysis (15). Mature human DMP-1 shares 61%-67% amino acid sequence identity with bovine, mouse, and rat DMP-1.

References

Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
Hirst, K.L. et al. (1997) Genomics 42:38.
He, G. et al. (2003) Nat. Mater. 2:552.
Gajjeraman, S. et al. (2007) J. Biol. Chem. 282:1193.
He, G. et al. (2005) Biochemistry 44:16140.
Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
Qin, C. et al. (2006 J. Biol. Chem. 281:8034.
Lu, Y. et al. (2007) Dev. Biol. 303:191.
Narayanan, K. et al. (2003) J. Biol. Chem. 278:17500.
Narayanan, K. et al. (2006) J. Biol. Chem. 281:19064.
Lorenz-Depiereux, B. et al. (2006) Nat. Genet. 38:1248.
Feng, J.Q. et al. (2006) Nat. Genet. 38:1310.
Fedarko, N.S. et al. (2004) FASEB J. 18:735.
Karadag, A. et al. (2005) Cancer Res. 65:11545.
Jain, A. et al. (2002) J. Biol. Chem. 277:13700.

Long Name

Dentin Matrix Protein 1

Entrez Gene IDs

1758 (Human); 13406 (Mouse); 25312 (Rat)

Alternate Names

ARHP; ARHR; dentin matrix acidic phosphoprotein 1; dentin matrix acidic phosphoprotein; Dentin matrix protein 1; DMP1; DMP-1

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Citation for Human DMP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1